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Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis
Jia, Zhihao1,4; Wang, Mengqiang1; Zhang, Huan1; Wang, Xiudan1,4; Lv, Zhao1,4; Wang, Lingling2; Song, Linsheng2,3
2018-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume74Pages:332-340
Corresponding AuthorSong, Linsheng(lshsong@qdio.ac.cn)
AbstractClip-domain serine proteinase is an important serine proteinase family involved in many biological processes, which is only found in invertebrates. In the present study, the full-length cDNA of a clip domain serine proteinase (designed as EsCDSP) gene was cloned from Chinese mitten crab Eriocheir sinensis using rapid amplification of cDNA ends (RACE) technique. It was of 1488 bp with an open reading frame (ORF) of 1134 bp encoding a polypeptide of 377 amino acids. There were a signal peptide, a clip domain, and a Tryp_SPc domain in the deduced amino acid sequence of EsCDSP. Highly conserved cysteine residues were identified in the clip domain and Tryp_SPc domain. EsCDSP shared similarities of 40%-61% with CDSPs from Penaeus monodon (ACP19562.1), Scylla paramamosain (CCW43200.1), Drosophila melanogaster (NP_649734.2) and Delia antiqua (AAW57295.1). It was clustered with other CDSPs from crabs in the phylogenetic tree. EsCDSP transcript was highly expressed in hemocytes and it could response to the stimulations of Vibro anguillarum and Pichia pastoris. rEsCDSP could activate proPO system and significantly increase the PO activity of HLS. In addition, rEsCDSP could bond to Aeromonas hydrophila, Vibro anguillarum and Vibro alginolyticus, and reduced the mortality rate causing by pathogen infection. All the results suggested that EsCDSP was an important immune response participator involved in activation of the proPO system of crab.
KeywordEriocheir sinensis Serine proteinase Clip domain Innate immunity proPO Microbial binding
DOI10.1016/j.fsi.2017.12.056
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation of China[31530069] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian high level talent innovation support program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University ; AoShan Talents Cultivation Program
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000427344200035
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158377
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, Linsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Zhihao,Wang, Mengqiang,Zhang, Huan,et al. Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:332-340.
APA Jia, Zhihao.,Wang, Mengqiang.,Zhang, Huan.,Wang, Xiudan.,Lv, Zhao.,...&Song, Linsheng.(2018).Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,74,332-340.
MLA Jia, Zhihao,et al."Identification of a clip domain serine proteinase involved in immune defense in Chinese mitten crab Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 74(2018):332-340.
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