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A novel GATA-like zinc finger transcription factor involving in hematopoiesis of Eriocheir sinensis
Li, Yannan1,2; Jia, Zhihao3; Yi, Qilin1,2; Song, Xiaorui1,2; Liu, Yu1,2; Jia, Yunke3; Wang, Lingling1,2; Song, Linsheng1,2
2018-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume74Pages:363-371
Corresponding AuthorSong, Linsheng(lshsong@qdio.ac.cn)
AbstractGATA transcription factor is a family of DNA-binding proteins that can recognize and bind to sequence of (A/T) GATA (A/G). In the present study, a GATA-like protein (named as EsGLP) was characterized from Eriocheir sinensis, including an 834 bp full length open reading frame of EsGLP, encoding a polypeptide of 277 amino acids. The deduced amino acid sequence of EsGLP contained one conserved GATA-type zinc finger of the form Cys-X2-Cys-X17-Cys-X2-Cys, with four cysteine sites. The EsGLP mRNA transcripts were mainly detected in the hematopoietic tissue, hepatopancreas and gonad. The recombinant EsGLP protein was prepared for the antibody production. The EsGLP protein was mainly distributed in the edge of lobules in the HPT and the cytoplasm of hemocytes. The mRNA transcripts of EsGLP in hemocytes were significantly decreased at 24 h (0.39-fold and 0.27-fold, p <.05) and 48 h (0.35-fold and 0.16-fold, p <.05) after LPS and Aeromonas hydrophila stimulation, respectively. However, one peak of EsGLP mRNA transcripts were recorded at 24 h (8.71-fold, p <.05) in HPT after A. hydrophila stimulation. The expression level of EsGLP mRNA in HPT was significantly up-regulated at 2 h, 2.5 h and 9 h (41.74-fold, 45.38-fold and 26.07-fold, p <.05) after exsanguination stimulation. When EsGLP gene expression was inhibited by the injection of double-stranded RNA, both the total hemocytes counts and the rate of EdU-positive hemocytes were significantly decreased (0.32-fold and 0.56-fold compared to that in control group, p <.05). All these results suggested that EsGLP was an important regulatory factor in E. sinensis which involved in the hemocytes generation and the immune response against invading pathogens.
KeywordEriocheir sinensis Hematopoiesis GATA-Like protein Hemocytes generation
DOI10.1016/j.fsi.2018.01.007
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation of China[31530069] ; AoShan Talents Cultivation Program ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning and Talented Scholars in Dalian Ocean University
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000427344200038
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158371
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, Linsheng
Affiliation1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Li, Yannan,Jia, Zhihao,Yi, Qilin,et al. A novel GATA-like zinc finger transcription factor involving in hematopoiesis of Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:363-371.
APA Li, Yannan.,Jia, Zhihao.,Yi, Qilin.,Song, Xiaorui.,Liu, Yu.,...&Song, Linsheng.(2018).A novel GATA-like zinc finger transcription factor involving in hematopoiesis of Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,74,363-371.
MLA Li, Yannan,et al."A novel GATA-like zinc finger transcription factor involving in hematopoiesis of Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 74(2018):363-371.
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