Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A novel GATA-like zinc finger transcription factor involving in hematopoiesis of Eriocheir sinensis | |
Li, Yannan1,2; Jia, Zhihao3; Yi, Qilin1,2; Song, Xiaorui1,2 ; Liu, Yu1,2; Jia, Yunke3; Wang, Lingling1,2; Song, Linsheng1,2
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| 2018-03-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| Volume | 74Pages:363-371 |
| Corresponding Author | Song, Linsheng(lshsong@qdio.ac.cn) |
| Abstract | GATA transcription factor is a family of DNA-binding proteins that can recognize and bind to sequence of (A/T) GATA (A/G). In the present study, a GATA-like protein (named as EsGLP) was characterized from Eriocheir sinensis, including an 834 bp full length open reading frame of EsGLP, encoding a polypeptide of 277 amino acids. The deduced amino acid sequence of EsGLP contained one conserved GATA-type zinc finger of the form Cys-X2-Cys-X17-Cys-X2-Cys, with four cysteine sites. The EsGLP mRNA transcripts were mainly detected in the hematopoietic tissue, hepatopancreas and gonad. The recombinant EsGLP protein was prepared for the antibody production. The EsGLP protein was mainly distributed in the edge of lobules in the HPT and the cytoplasm of hemocytes. The mRNA transcripts of EsGLP in hemocytes were significantly decreased at 24 h (0.39-fold and 0.27-fold, p <.05) and 48 h (0.35-fold and 0.16-fold, p <.05) after LPS and Aeromonas hydrophila stimulation, respectively. However, one peak of EsGLP mRNA transcripts were recorded at 24 h (8.71-fold, p <.05) in HPT after A. hydrophila stimulation. The expression level of EsGLP mRNA in HPT was significantly up-regulated at 2 h, 2.5 h and 9 h (41.74-fold, 45.38-fold and 26.07-fold, p <.05) after exsanguination stimulation. When EsGLP gene expression was inhibited by the injection of double-stranded RNA, both the total hemocytes counts and the rate of EdU-positive hemocytes were significantly decreased (0.32-fold and 0.56-fold compared to that in control group, p <.05). All these results suggested that EsGLP was an important regulatory factor in E. sinensis which involved in the hemocytes generation and the immune response against invading pathogens. |
| Keyword | Eriocheir sinensis Hematopoiesis GATA-Like protein Hemocytes generation |
| DOI | 10.1016/j.fsi.2018.01.007 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Science Foundation of China[31530069] ; AoShan Talents Cultivation Program ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning and Talented Scholars in Dalian Ocean University |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS Subject | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000427344200038 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158371 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Song, Linsheng |
| Affiliation | 1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Yannan,Jia, Zhihao,Yi, Qilin,et al. A novel GATA-like zinc finger transcription factor involving in hematopoiesis of Eriocheir sinensis[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:363-371. |
| APA | Li, Yannan.,Jia, Zhihao.,Yi, Qilin.,Song, Xiaorui.,Liu, Yu.,...&Song, Linsheng.(2018).A novel GATA-like zinc finger transcription factor involving in hematopoiesis of Eriocheir sinensis.FISH & SHELLFISH IMMUNOLOGY,74,363-371. |
| MLA | Li, Yannan,et al."A novel GATA-like zinc finger transcription factor involving in hematopoiesis of Eriocheir sinensis".FISH & SHELLFISH IMMUNOLOGY 74(2018):363-371. |
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| A novel GATA-like zi（1103KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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