IOCAS-IR  > 实验海洋生物学重点实验室
A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas
Jia, Yunke1,3; Yang, Bin1,3; Dong, Wenjing1,3; Liu, Zhaoqun4; Lv, Zhao1,3; Jia, Zhihao1,3; Qiu, Limei1; Wang, Lingling2,4; Song, Linsheng2,4
2018-05-01
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
卷号82页码:83-93
通讯作者Wang, Lingling(wanglingling@dlou.edu.cn) ; Song, Linsheng()
摘要Serotonin receptors, including ligand-gated ion channel (LGICs) and G protein-coupled receptors (GPCR), play vital roles in modulating physiological processes and immunoreaction. In the present study, a homologue of serotonin (5-HT) receptor was identified from oyster Crassostrea gigas (designated Cg5-HTR1). Its open reading frame (ORF) was of 1239 bp, encoding-a polypeptide of 412 amino acids with a seven transmembrane region. Cg5-HTR-1 shared high similarity with the 5-HTRs from other animals. The cAMP contents in HEK293T cells decreased significantly after Cg5-HTR-1 transfection and 5-HT incubation (p < .05), while blocking Cg5-HTR-1 with specific receptor antagonist reversed this downtrend. The intracellular Ca2+ concentrations increased significantly (p < .05) after cell transfection and 5-HT incubation, and the antagonist treatment also arrested this process. Cg5-HTR-1 transcripts were widely distributed in various tissues, with the highest level in hepatopancreas and lowest level in mantle and gill. The mRNA expression of Cg5-HTR-1 in hemocyte increased significantly after lipopolysaccharide (LPS) stimulation and reached the peak level (6.47-fold, p < .05) at 6 h post treatment. The inhibition of Cg5-HTR-1 significantly reduced the expression of tumor necrosis factor (TNF) mRNA in hemocyte, down-regulated the superoxide dismutase (SOD) activity in serum, and induced the apoptosis of hemocyte (p < .05). These results suggested that Cg5-HTR-1 was a novel member of 5-HT1 receptor family and it mediated serotonergic immunomodulation on both cellular and humoral immune responses. (C) 2018 Elsevier Ltd. All rights reserved.
关键词Serotonin Serotonin receptor 1A Cellular immune response Humoral immune response Crassostrea gigas
DOI10.1016/j.dci.2017.12.029
收录类别SCI
语种英语
资助项目National Science Foundation of China[U1706204] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Earmarked Fund from Modern Agro-industry Technology Research System[CARS-49] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Aoshan Talent Cultivation Program ; National Science Foundation of China[U1706204] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Earmarked Fund from Modern Agro-industry Technology Research System[CARS-49] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Aoshan Talent Cultivation Program
WOS研究方向Fisheries ; Immunology ; Zoology
WOS类目Fisheries ; Immunology ; Zoology
WOS记录号WOS:000427668700009
出版者ELSEVIER SCI LTD
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/158367
专题实验海洋生物学重点实验室
通讯作者Wang, Lingling; Song, Linsheng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
第一作者单位实验海洋生物学重点实验室
推荐引用方式
GB/T 7714
Jia, Yunke,Yang, Bin,Dong, Wenjing,et al. A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,82:83-93.
APA Jia, Yunke.,Yang, Bin.,Dong, Wenjing.,Liu, Zhaoqun.,Lv, Zhao.,...&Song, Linsheng.(2018).A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,82,83-93.
MLA Jia, Yunke,et al."A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 82(2018):83-93.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
A serotonin receptor(2259KB)期刊论文出版稿限制开放CC BY-NC-SA浏览
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Jia, Yunke]的文章
[Yang, Bin]的文章
[Dong, Wenjing]的文章
百度学术
百度学术中相似的文章
[Jia, Yunke]的文章
[Yang, Bin]的文章
[Dong, Wenjing]的文章
必应学术
必应学术中相似的文章
[Jia, Yunke]的文章
[Yang, Bin]的文章
[Dong, Wenjing]的文章
相关权益政策
暂无数据
收藏/分享
文件名: A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。