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A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas
Jia, Yunke1,3; Yang, Bin1,3; Dong, Wenjing1,3; Liu, Zhaoqun4; Lv, Zhao1,3; Jia, Zhihao1,3; Qiu, Limei1; Wang, Lingling2,4; Song, Linsheng2,4
Corresponding AuthorWang, Lingling( ; Song, Linsheng()
AbstractSerotonin receptors, including ligand-gated ion channel (LGICs) and G protein-coupled receptors (GPCR), play vital roles in modulating physiological processes and immunoreaction. In the present study, a homologue of serotonin (5-HT) receptor was identified from oyster Crassostrea gigas (designated Cg5-HTR1). Its open reading frame (ORF) was of 1239 bp, encoding-a polypeptide of 412 amino acids with a seven transmembrane region. Cg5-HTR-1 shared high similarity with the 5-HTRs from other animals. The cAMP contents in HEK293T cells decreased significantly after Cg5-HTR-1 transfection and 5-HT incubation (p < .05), while blocking Cg5-HTR-1 with specific receptor antagonist reversed this downtrend. The intracellular Ca2+ concentrations increased significantly (p < .05) after cell transfection and 5-HT incubation, and the antagonist treatment also arrested this process. Cg5-HTR-1 transcripts were widely distributed in various tissues, with the highest level in hepatopancreas and lowest level in mantle and gill. The mRNA expression of Cg5-HTR-1 in hemocyte increased significantly after lipopolysaccharide (LPS) stimulation and reached the peak level (6.47-fold, p < .05) at 6 h post treatment. The inhibition of Cg5-HTR-1 significantly reduced the expression of tumor necrosis factor (TNF) mRNA in hemocyte, down-regulated the superoxide dismutase (SOD) activity in serum, and induced the apoptosis of hemocyte (p < .05). These results suggested that Cg5-HTR-1 was a novel member of 5-HT1 receptor family and it mediated serotonergic immunomodulation on both cellular and humoral immune responses. (C) 2018 Elsevier Ltd. All rights reserved.
KeywordSerotonin Serotonin receptor 1A Cellular immune response Humoral immune response Crassostrea gigas
Indexed BySCI
Funding ProjectNational Science Foundation of China[U1706204] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Earmarked Fund from Modern Agro-industry Technology Research System[CARS-49] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Aoshan Talent Cultivation Program
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000427668700009
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorWang, Lingling; Song, Linsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jia, Yunke,Yang, Bin,Dong, Wenjing,et al. A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,82:83-93.
APA Jia, Yunke.,Yang, Bin.,Dong, Wenjing.,Liu, Zhaoqun.,Lv, Zhao.,...&Song, Linsheng.(2018).A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,82,83-93.
MLA Jia, Yunke,et al."A serotonin receptor (Cg5-HTR-1) mediating immune response in oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 82(2018):83-93.
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