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Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus
Fu, Yuanshuai1; Gao, Lina1; Shi, Zhiyi1; You, Feng2; Zhang, Junling1; Li, Wenjuan1
2018-04-01
发表期刊FISH PHYSIOLOGY AND BIOCHEMISTRY
ISSN0920-1742
卷号44期号:2页码:451-463
通讯作者Shi, Zhiyi(zyshi@shou.edu.cn)
摘要Heterochronic lin-28 is a conserved RNA-binding protein that plays a key role in the timing of developmental events in organisms. As a crucial heterochronic gene, the protein controls developmental events of the second of four larval stages in Caenorhabditi elegans. Heterochronic let-7 miRNAs are often present in various species and highly conserved in sequence and biological function and are required for various biological processes. Previous studies showed that ten let-7 miRNAs were identified in the Japanese flounder (Paralichthys olivaceus) and that they were primarily expressed during metamorphosis. In this study, we clone and characterize the lin-28a gene from P. olivaceus and exhibit its dynamic expression pattern at different developmental stages and various adult tissues. The results show that the P. olivaceus lin-28a gene has high sequence similarity with other species and is highly expressed in the embryonic stage but weakly expressed in the larval stage. In addition, lin-28a overexpression causes cell proliferation and significantly promotes the levels of pre-let-7a and pre-let-7d while markedly depressing let-7a and let-7d expression in FEC (Flounder Embryonic Cell), which indicate that lin-28 possibly blocks the maturation of let-7 miRNAs. Additionally, lin-28a is identified as a target gene of let-7 miRNAs, and let-7 miRNAs directly regulate lin-28a expression by targeting its 3' UTR. Taken together, lin-28a along with let-7 miRNA participates in a lin-28/let-7 axis pathway that regulates cell division and timing of embryonic and metamorphic events in P. olivaceus.
关键词P. olivaceus lin-28a let-7 miRNAs Embryonic development Metamorphosis
DOI10.1007/s10695-017-0445-1
收录类别SCI
语种英语
资助项目Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2015No01] ; National Natural Science Foundation of China[41506159] ; National Natural Science Foundation of China[41306128] ; Natural Science Foundation of Shanghai[15ZR1420600] ; Innovation Programme of Shanghai Municipal Education Commission[15ZZ083] ; Science and Technology Development Foundation of Shanghai Ocean University[A2-0203-00-100204] ; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences[KF2015No01] ; National Natural Science Foundation of China[41506159] ; National Natural Science Foundation of China[41306128] ; Natural Science Foundation of Shanghai[15ZR1420600] ; Innovation Programme of Shanghai Municipal Education Commission[15ZZ083] ; Science and Technology Development Foundation of Shanghai Ocean University[A2-0203-00-100204]
WOS研究方向Biochemistry & Molecular Biology ; Fisheries ; Physiology
WOS类目Biochemistry & Molecular Biology ; Fisheries ; Physiology
WOS记录号WOS:000428055300003
出版者SPRINGER
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/158358
专题实验海洋生物学重点实验室
通讯作者Shi, Zhiyi
作者单位1.Shanghai Ocean Univ, Minist Agr, Key Lab Genet Resources Freshwater Aquaculture &, 999 Hu Cheng Huan Rd, Shanghai 201306, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
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Fu, Yuanshuai,Gao, Lina,Shi, Zhiyi,et al. Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2018,44(2):451-463.
APA Fu, Yuanshuai,Gao, Lina,Shi, Zhiyi,You, Feng,Zhang, Junling,&Li, Wenjuan.(2018).Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus.FISH PHYSIOLOGY AND BIOCHEMISTRY,44(2),451-463.
MLA Fu, Yuanshuai,et al."Characterization and expression of lin-28a involved in lin28/let-7signal pathway during early development of P-olivaceus".FISH PHYSIOLOGY AND BIOCHEMISTRY 44.2(2018):451-463.
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