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| Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis | |
| Li, Yi-qun1,2,3; Sun, Li1,2; Li, Jun4,5 | |
| 2018-05-01 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| ISSN | 0145-305X |
| Volume | 82Pages:31-38 |
| Corresponding Author | Sun, Li(lsun@qdio.ac.cn) ; Li, Jun(jli@lssu.edu) |
| Abstract | Increasing evidence has demonstrated support for the endocytic capacities of teleost B cells. In the present study, the ability of turbot IgM(+) B cells to ingest microspheres of different sizes and the corresponding internalization pathways were investigated. The results showed that IgM(+) B cells exhibited relatively high endocytic capacities for 0.5 mu m and 1 mu m latex beads, and that different mechanisms were employed for IgM(+) and IgM(-) cells to uptake 0.5 gm and 1 gm beads. For 0.5 gm beads, IgM(+) B cells apparently employed macropinocytosis-dependent endocytic pathway, whereas IgM(-) cells utilized a different process involving both clathrin-and caveolae-mediated pathways. For the uptake of 1 mu m beads, IgM(+) cells relied mainly on macropinocytosis and partially on caveolae-mediated pathway, while IgM(-) cells utilized the routes similar to that of internalizing 0.5 mu m beads. Consistently, the internalized microspheres were co-localized with high-molecular-mass dextran in IgM(+) phagocytic cells. In addition to latex beads, IgM(+) B cells could also ingest inactivated bacteria predominately through macropinocytosis and caveolae-mediated endocytosis. These results collectively indicated that macropinocytosis is principally responsible for particle uptake by turbot IgM(+) B cells. (C) 2018 Elsevier Ltd. All rights reserved. |
| Keyword | Scophthalmus maximus IgM(+) cells Endocytosis Macropinocytosis |
| DOI | 10.1016/j.dci.2017.12.028 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31528019] ; AoShan Talents Program from Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Fisheries ; Immunology ; Zoology |
| WOS Subject | Fisheries ; Immunology ; Zoology |
| WOS ID | WOS:000427668700003 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158342 |
| Collection | 中国科学院海洋研究所 |
| Corresponding Author | Sun, Li; Li, Jun |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 5.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI 49783 USA |
| First Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Li, Yi-qun,Sun, Li,Li, Jun. Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,82:31-38. |
| APA | Li, Yi-qun,Sun, Li,&Li, Jun.(2018).Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,82,31-38. |
| MLA | Li, Yi-qun,et al."Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 82(2018):31-38. |
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| File Name/Size | DocType | Version | Access | License | ||
| Internalization of l（2306KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | Application Full Text | |
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