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Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation
Wang, Mengqiang1,3; Liu, Mei1; Wang, Baojie1; Jiang, Keyong1; Jia, Zhihao1,4; Wang, Lingling5; Wang, Lei1,2
2018-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume74Pages:540-550
Corresponding AuthorWang, Lingling(wanglingling@dlou.edu.cn) ; Wang, Lei(wanglei@qdio.ac.cn)
AbstractAs marine invertebrates, oysters lack adaptive immunity and employ innate immunity as the front line and almost the solo defense mechanism to protect them against invaders. Accumulating research achievements demonstrated that exosomes could act as innate immune effectors that contribute to host defense mechanism. To better understand the immune functions of exosomes in Crassostrea gigas against bacterial stimulation, RNA-Seq was applied to explore the global expression changes of exosomes in oyster after Staphylococcus aureus and Vibrio splendidus stimulation. Totally 171573691 single end raw reads were yielded via Ion Torrent Proton sequencing, which were trimmed into 121988325 clean reads, and then 1505 abundant exosomal shuttle mRNAs (esmRNAs) were identified. Gene ontology (GO) analysis revealed that these abundant esmRNAs could be categorized into 15 cellular components, 12 molecular functions and 21 biological processes, and these abundant esmRNAs were mapped onto 62 biological signaling pathways by KEGG. In total, 68 significant differentially expressed genes (DEGs, Fold change >= 2, Q-value < 0.05) were identified between S. aureus stimulated group and control group, including 21 up-regulated and 47 down regulated ones. While 99 significant DEGs between V. splendidus challenged group and control group were identified, including 42 up-regulated and 57 down-regulated ones. To validate the transcriptomic data, 24 DEGs were randomly selected and confirmed via quantitative real-time PCR (qRT-PCR) and the results showed that their expression patterns agreed well with the RNA-Seq analysis. This study would enrich the C. gigas transcriptome database and provide insight into the immune functions of oyster exosomes against bacterial infection.
KeywordCrassostrea gigas Exosomes Extracellular vesicles Innate immunity
DOI10.1016/j.fsi.2018.01.017
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41676134] ; National Natural Science Foundation of China[41306156] ; National Natural Science Foundation of China[31402337] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; National High Technology Research and Development Program of China[2014AA093501]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000427344200058
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158120
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lingling; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Dalian Ocean Univ, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Mengqiang,Liu, Mei,Wang, Baojie,et al. Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:540-550.
APA Wang, Mengqiang.,Liu, Mei.,Wang, Baojie.,Jiang, Keyong.,Jia, Zhihao.,...&Wang, Lei.(2018).Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation.FISH & SHELLFISH IMMUNOLOGY,74,540-550.
MLA Wang, Mengqiang,et al."Transcriptomic analysis of exosomal shuttle mRNA in Pacific oyster Crassostrea gigas during bacterial stimulation".FISH & SHELLFISH IMMUNOLOGY 74(2018):540-550.
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