Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei | |
Zhang, Xiaoxi1,2,3; Zhang, Xiaojun1,2; Yuan, Jianbo1,2; Du, Jiangli1,2,3 ; Li, Fuhua1,2; Xiang, Jianhai1,2
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| 2018-04-01 | |
| Source Publication | MOLECULAR GENETICS AND GENOMICS
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| ISSN | 1617-4615 |
| Volume | 293Issue:2Pages:479-493 |
| Corresponding Author | Zhang, Xiaoxi(xiaoxizhang11@gmail.com) |
| Abstract | Actin is a multi-functional gene family that can be divided into muscle-type actins and non-muscle-type actins. In this study, 37 unigenes encoding actins were identified from RNA-Seq data of Pacific white shrimp, Litopenaeus vannamei. According to phylogenetic analysis, four and three cDNAs belong to cytoplasmic- and heart-type actins and were named LvActinCT and LvActinHT, respectively. 10 cDNAs belong to the slow-type skeletal muscle actins, and 18 belong to the fast-type skeletal muscle actins; they were designated LvActinSSK and LvActinFSK, respectively. Some muscle actin genes formed gene clusters in the genome. Multiple alternative transcription starts sites (ATSSs) were found for LvActinCT1. Based on the early developmental expression profile, almost all LvActins were highly expressed between the early limb bud and post-larval stages. Using LvActinSSK5 as probes, slow-type muscle was localized in pleopod muscle and superficial ventral muscle. We also found three actin genes that were down-regulated in the hemocytes of white spot syndrome virus (WSSV)- and Vibrio parahaemolyticus-infected L. vannamei. This study provides valuable information on the actin gene structure of shrimp, furthers our understanding of the shrimp muscle system and helps us develop strategies for disease control and sustainable shrimp farming. |
| Keyword | Actin gene Litopenaeus vannamei RNA-Seq Muscle development Disease control |
| DOI | 10.1007/s00438-017-1397-y |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31672632] ; National Natural Science Foundation of China[41506189] ; National Natural Science Foundation of China[41376165] ; China Agriculture Research System-47[CARS-47] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-3] |
| WOS Research Area | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS Subject | Biochemistry & Molecular Biology ; Genetics & Heredity |
| WOS ID | WOS:000427630400014 |
| Publisher | SPRINGER HEIDELBERG |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158117 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Zhang, Xiaoxi |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,et al. Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei[J]. MOLECULAR GENETICS AND GENOMICS,2018,293(2):479-493. |
| APA | Zhang, Xiaoxi,Zhang, Xiaojun,Yuan, Jianbo,Du, Jiangli,Li, Fuhua,&Xiang, Jianhai.(2018).Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei.MOLECULAR GENETICS AND GENOMICS,293(2),479-493. |
| MLA | Zhang, Xiaoxi,et al."Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei".MOLECULAR GENETICS AND GENOMICS 293.2(2018):479-493. |
| Files in This Item: | ||||||
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| Actin genes and thei（5635KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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