Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Isolation and characterization of Ulva prolifera actin 1 gene and function verification of the 5 ' flanking region as a strong promoter | |
Wu, Chunhui1,2,3 ; Jiang, Peng1,3; Guo, Yang1,2,3; Liu, Jianguo1,3; Zhao, Jin1,3; Fu, Huihui1,3
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| 2018 | |
| 发表期刊 | BIOENGINEERED
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| ISSN | 2165-5979 |
| 卷号 | 9期号:1页码:124-133 |
| 摘要 | Ulva prolifera is a green macroalgae with an extremely high growth rate that can accumulate biomass with considerable protein content. To set up an available seaweed expression system, a prior step is to isolate endogenous and strong constitutive promoters. For this reason, the full-length genomic actin1 gene from U. prolifera (Upactin1) was cloned and its 5' flanking sequence was obtained by genome walking. The Upactin1 open reading frame consisted of 1134 nucleotides encoding 377 amino acid residues. Besides 4 exons and 3 introns in the coding region, an extra leader intron was identified in the 5' untranslated region. According to quantitative GUS assays based on transient expression, the promoter activity of the Upactin1 5' flanking region was found to be several times higher than that of the widely-used cauliflower mosaic virus 35S (CaMV35S) in all tested species of Ulva. In addition, precise deletion of the leader intron led to a significant decrease of promoter strength in U. prolifera, and almost entire loss of strength in U. linza and U. pertusa. To our knowledge, this is the first report to prove function of a leader intron in algae. The 5' flanking region of Upactin1 was shown to be a much stronger promoter than the foreign CaMV35S, and its activity was highly dependent on the presence of the leader intron. We propose that the Upactin1 promoter could serve as an endogenous and strong constitutive element for genetic engineering of U. prolifera. |
| 关键词 | actin endogenous promoter gene structure leader intron quantitative GUS assay Ulva prolifera |
| DOI | 10.1080/21655979.2017.1325041 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National High Technology Research and Development Program of China[2014AA093501] ; Chinese Academy of Sciences under Strategic Priority Research Program[XDA11020303] ; Chinese Academy of Sciences under Strategic Priority Research Program[XDA11020304] ; Qingdao National Laboratory for Marine Science and Technology under Scientific and Technological Innovation Project[2016ASKJ02-1] ; Qingdao National Laboratory for Marine Science and Technology under Scientific and Technological Innovation Project[2016ASKJ02-1] ; Chinese Academy of Sciences under Strategic Priority Research Program[XDA11020304] ; Chinese Academy of Sciences under Strategic Priority Research Program[XDA11020303] ; National High Technology Research and Development Program of China[2014AA093501] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000425053900003 |
| 出版者 | TAYLOR & FRANCIS INC |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 版本 | 出版稿 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158086 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Jiang, Peng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Wu, Chunhui,Jiang, Peng,Guo, Yang,et al. Isolation and characterization of Ulva prolifera actin 1 gene and function verification of the 5 ' flanking region as a strong promoter[J]. BIOENGINEERED,2018,9(1):124-133. |
| APA | Wu, Chunhui,Jiang, Peng,Guo, Yang,Liu, Jianguo,Zhao, Jin,&Fu, Huihui.(2018).Isolation and characterization of Ulva prolifera actin 1 gene and function verification of the 5 ' flanking region as a strong promoter.BIOENGINEERED,9(1),124-133. |
| MLA | Wu, Chunhui,et al."Isolation and characterization of Ulva prolifera actin 1 gene and function verification of the 5 ' flanking region as a strong promoter".BIOENGINEERED 9.1(2018):124-133. |
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