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Isolation and characterization of Ulva prolifera actin 1 gene and function verification of the 5 ' flanking region as a strong promoter
Wu, Chunhui1,2,3; Jiang, Peng1,3; Guo, Yang1,2,3; Liu, Jianguo1,3; Zhao, Jin1,3; Fu, Huihui1,3
2018
Source PublicationBIOENGINEERED
ISSN2165-5979
Volume9Issue:1Pages:124-133
Corresponding AuthorJiang, Peng(jiangpeng@qdio.ac.cn)
AbstractUlva prolifera is a green macroalgae with an extremely high growth rate that can accumulate biomass with considerable protein content. To set up an available seaweed expression system, a prior step is to isolate endogenous and strong constitutive promoters. For this reason, the full-length genomic actin1 gene from U. prolifera (Upactin1) was cloned and its 5' flanking sequence was obtained by genome walking. The Upactin1 open reading frame consisted of 1134 nucleotides encoding 377 amino acid residues. Besides 4 exons and 3 introns in the coding region, an extra leader intron was identified in the 5' untranslated region. According to quantitative GUS assays based on transient expression, the promoter activity of the Upactin1 5' flanking region was found to be several times higher than that of the widely-used cauliflower mosaic virus 35S (CaMV35S) in all tested species of Ulva. In addition, precise deletion of the leader intron led to a significant decrease of promoter strength in U. prolifera, and almost entire loss of strength in U. linza and U. pertusa. To our knowledge, this is the first report to prove function of a leader intron in algae. The 5' flanking region of Upactin1 was shown to be a much stronger promoter than the foreign CaMV35S, and its activity was highly dependent on the presence of the leader intron. We propose that the Upactin1 promoter could serve as an endogenous and strong constitutive element for genetic engineering of U. prolifera.
Keywordactin endogenous promoter gene structure leader intron quantitative GUS assay Ulva prolifera
DOI10.1080/21655979.2017.1325041
Indexed BySCI
Language英语
Funding ProjectQingdao National Laboratory for Marine Science and Technology under Scientific and Technological Innovation Project[2016ASKJ02-1] ; Chinese Academy of Sciences under Strategic Priority Research Program[XDA11020304] ; Chinese Academy of Sciences under Strategic Priority Research Program[XDA11020303] ; National High Technology Research and Development Program of China[2014AA093501]
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000425053900003
PublisherTAYLOR & FRANCIS INC
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158086
Collection实验海洋生物学重点实验室
Corresponding AuthorJiang, Peng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Chunhui,Jiang, Peng,Guo, Yang,et al. Isolation and characterization of Ulva prolifera actin 1 gene and function verification of the 5 ' flanking region as a strong promoter[J]. BIOENGINEERED,2018,9(1):124-133.
APA Wu, Chunhui,Jiang, Peng,Guo, Yang,Liu, Jianguo,Zhao, Jin,&Fu, Huihui.(2018).Isolation and characterization of Ulva prolifera actin 1 gene and function verification of the 5 ' flanking region as a strong promoter.BIOENGINEERED,9(1),124-133.
MLA Wu, Chunhui,et al."Isolation and characterization of Ulva prolifera actin 1 gene and function verification of the 5 ' flanking region as a strong promoter".BIOENGINEERED 9.1(2018):124-133.
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