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Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera
Wang, Xueqin; Yu, Huahua; Xing, Ronge; Chen, Xiaolin; Liu, Song; Li, Pengcheng1
2018-02-15
Source PublicationPEERJ
ISSN2167-8359
Volume6Pages:21
Corresponding AuthorLi, Pengcheng(pcli@qdio.ac.cn)
AbstractMackerel (Pneumatophorus japonicus) viscera contain large amount of protein. We used five proteases to hydrolyze the viscera, and the hydrolysate treated by neutrase exhibited the highest nitrogen recovery (NR). Then we optimized the preparation conditions for mackerel viscera hydrolysate (MVH) by response surface methodology and investigated the antioxidant activity of MVH. The optimal conditions were as follows: enzyme concentration of 1,762.87 U/g, pH of 6.76, temperature of 43.75 degrees C, extraction time of 6.0 h and water/material ratio of 20.37 (v/w), and the maximum NR was 37.84%. Furthermore, the molecular weight distribution of MVH was almost below 3,000 Da determined by TSK G2000 SWXL gel filtration chromatography, and the MVH exhibited good antioxidant activities in various in vitro assays, including DPPH radical, hydroxyl radical and superoxide anion scavenging activities, reducing power and similar effectivelness as butylated hydroxytoluene and Vitamin E to inhibit lipid peroxidation. The results suggested that MVH could be used as a potential source of antioxidant peptide in food industries.
KeywordMackerel viscera Protease Response surface methodology Antioxidant activity
DOI10.7717/peerj.4373
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41506175] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; Qingdao Science and Technology Project[17-3-3-21-nsh] ; Chinese Academy of Sciences[KFZD-SW-106] ; Science and Technology Development Plan Project of Shandong Province[2016YYSP010]
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000425362200006
PublisherPEERJ INC
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158073
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Pengcheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xueqin,Yu, Huahua,Xing, Ronge,et al. Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera[J]. PEERJ,2018,6:21.
APA Wang, Xueqin,Yu, Huahua,Xing, Ronge,Chen, Xiaolin,Liu, Song,&Li, Pengcheng.(2018).Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera.PEERJ,6,21.
MLA Wang, Xueqin,et al."Optimization of antioxidative peptides from mackerel (Pneumatophorus japonicus) viscera".PEERJ 6(2018):21.
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