Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| CPAP3 proteins in the mineralized cuticle of a decapod crustacean | |
Abehsera, Shai1,2; Zaccai, Shir1; Mittelman, Binyamin1,2; Glazer, Lilah4; Weil, Simy1,2; Khalaila, Isam2,3; Davidov, Geula1,2; Bitton, Ronit1,2; Zarivach, Raz1,2; Li, Shihao5; Li, Fuhua5; Xiang, Jianhai5 ; Manor, Rivka1,2; Aflalo, Eliahu D.1,2; Sagi, Amir1,2
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| 2018-02-05 | |
| Source Publication | SCIENTIFIC REPORTS
 |
| ISSN | 2045-2322 |
| Volume | 8Pages:12 |
| Corresponding Author | Sagi, Amir(sagia@bgu.ac.il) |
| Abstract | The pancrustacean theory groups crustaceans and hexapods (once thought to comprise separate clades within the Arthropoda) into a single clade. A key feature common to all pancrustaceans is their chitinous exoskeleton, with a major contribution by cuticular proteins. Among these, are the CPAP3' s, a family of cuticular proteins, first identified in the hexapod Drosophila melanogaster and characterized by an N-terminal signaling peptide and three chitin-binding domains. In this study, CPAP3 proteins were mined from a transcriptomic library of a decapod crustacean, the crayfish Cherax quadricarinatus. Phylogenetic analysis of other CPAP3 proteins from hexapods and other crustaceans showed a high degree of conservation. Characterization of the crayfish proteins, designated CqCPAP3's, suggested a major role for CPAP3'sin cuticle formation. Loss-of-function experiments using RNAi supported such a notion by demonstrating crucial roles for several CqCPAP3 proteins during molting. A putative mode of action for the CqCPAP3 proteins-theoretically binding three chitin strands-was suggested by the structural data obtained from a representative recombinant CqCPAP3. The similarities between the CqCPAP3 proteins and their hexapod homologues further demonstrated common genetic and proteinaceous features of cuticle formation in pancrustaceans, thereby reinforcing the linkage between these two highly important phylogenetic groups. |
| DOI | 10.1038/s41598-018-20835-x |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Israel Science Foundation[613/13] ; Israel Science Foundation ; National Natural Science Foundation of China[605/14] ; National Institute for Biotechnology in the Negev (NIBN) |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000424087700102 |
| Publisher | NATURE PUBLISHING GROUP |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158070 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sagi, Amir |
| Affiliation | 1.Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel 2.Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, Beer Sheva, Israel 3.Ben Gurion Univ Negev, Dept Biotechnol Engn, Beer Sheva, Israel 4.Duke Univ, Med Ctr, Dept Psychiat & Behav Sci, Durham, NC USA 5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| Recommended Citation GB/T 7714 | Abehsera, Shai,Zaccai, Shir,Mittelman, Binyamin,et al. CPAP3 proteins in the mineralized cuticle of a decapod crustacean[J]. SCIENTIFIC REPORTS,2018,8:12. |
| APA | Abehsera, Shai.,Zaccai, Shir.,Mittelman, Binyamin.,Glazer, Lilah.,Weil, Simy.,...&Sagi, Amir.(2018).CPAP3 proteins in the mineralized cuticle of a decapod crustacean.SCIENTIFIC REPORTS,8,12. |
| MLA | Abehsera, Shai,et al."CPAP3 proteins in the mineralized cuticle of a decapod crustacean".SCIENTIFIC REPORTS 8(2018):12. |
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