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CPAP3 proteins in the mineralized cuticle of a decapod crustacean
Abehsera, Shai1,2; Zaccai, Shir1; Mittelman, Binyamin1,2; Glazer, Lilah4; Weil, Simy1,2; Khalaila, Isam2,3; Davidov, Geula1,2; Bitton, Ronit1,2; Zarivach, Raz1,2; Li, Shihao5; Li, Fuhua5; Xiang, Jianhai5; Manor, Rivka1,2; Aflalo, Eliahu D.1,2; Sagi, Amir1,2
2018-02-05
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume8Pages:12
Corresponding AuthorSagi, Amir(sagia@bgu.ac.il)
AbstractThe pancrustacean theory groups crustaceans and hexapods (once thought to comprise separate clades within the Arthropoda) into a single clade. A key feature common to all pancrustaceans is their chitinous exoskeleton, with a major contribution by cuticular proteins. Among these, are the CPAP3' s, a family of cuticular proteins, first identified in the hexapod Drosophila melanogaster and characterized by an N-terminal signaling peptide and three chitin-binding domains. In this study, CPAP3 proteins were mined from a transcriptomic library of a decapod crustacean, the crayfish Cherax quadricarinatus. Phylogenetic analysis of other CPAP3 proteins from hexapods and other crustaceans showed a high degree of conservation. Characterization of the crayfish proteins, designated CqCPAP3's, suggested a major role for CPAP3'sin cuticle formation. Loss-of-function experiments using RNAi supported such a notion by demonstrating crucial roles for several CqCPAP3 proteins during molting. A putative mode of action for the CqCPAP3 proteins-theoretically binding three chitin strands-was suggested by the structural data obtained from a representative recombinant CqCPAP3. The similarities between the CqCPAP3 proteins and their hexapod homologues further demonstrated common genetic and proteinaceous features of cuticle formation in pancrustaceans, thereby reinforcing the linkage between these two highly important phylogenetic groups.
DOI10.1038/s41598-018-20835-x
Indexed BySCI
Language英语
Funding ProjectIsrael Science Foundation[613/13] ; Israel Science Foundation ; National Natural Science Foundation of China[605/14] ; National Institute for Biotechnology in the Negev (NIBN)
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000424087700102
PublisherNATURE PUBLISHING GROUP
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158070
Collection实验海洋生物学重点实验室
Corresponding AuthorSagi, Amir
Affiliation1.Ben Gurion Univ Negev, Dept Life Sci, Beer Sheva, Israel
2.Ben Gurion Univ Negev, Natl Inst Biotechnol Negev, Beer Sheva, Israel
3.Ben Gurion Univ Negev, Dept Biotechnol Engn, Beer Sheva, Israel
4.Duke Univ, Med Ctr, Dept Psychiat & Behav Sci, Durham, NC USA
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Abehsera, Shai,Zaccai, Shir,Mittelman, Binyamin,et al. CPAP3 proteins in the mineralized cuticle of a decapod crustacean[J]. SCIENTIFIC REPORTS,2018,8:12.
APA Abehsera, Shai.,Zaccai, Shir.,Mittelman, Binyamin.,Glazer, Lilah.,Weil, Simy.,...&Sagi, Amir.(2018).CPAP3 proteins in the mineralized cuticle of a decapod crustacean.SCIENTIFIC REPORTS,8,12.
MLA Abehsera, Shai,et al."CPAP3 proteins in the mineralized cuticle of a decapod crustacean".SCIENTIFIC REPORTS 8(2018):12.
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