A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection

doi:10.1016/j.dci.2017.12.018

IOCAS-IR > 实验海洋生物学重点实验室

	A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection
	Yang, Feifei 1; Li, Shihao 1; Li, Fuhua 1,2; Xiang, Jianhai1,2
	2018-04-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	81 页码:303-311
通讯作者	Li, Fuhua(fhli@qdio.ac.cn)
摘要	White spot syndrome virus (WSSV) is a major viral pathogen in global shrimp farming, causing huge economic damage. Through penetrating the outer surface of the target tissues, WSSV enters into the cells of the target tissue to complete the replication process in the host. In the present study, a cuticle protein gene from Litopenaeus vannamei, designated as LvAMP13.4, was identified and proved to be involved in WSSV invasion. The deduced amino acid sequence of LvAMP13.4 contained a signal peptide and a conserved chitin-binding domain type 4 (ChBD4). This cuticle protein gene was mainly expressed in stomach, gill and epidermis. The expression level of LvAMP13.4 was significantly changed during WSSV infection. Silencing of LvAMP13.4 by dsRNA interference apparently reduced the mortality rate and the WSSV copy number in shrimp upon WSSV infection. Furthermore, yeast two-hybrid system and Co-IP assay were performed to confirm that LvAMP13.4 could interact with the major envelop protein VP24 of WSSV. These data indicated that LvAMP13.4 was involved in the invasion process of WSSV through interaction with VP24. The present results could provide new insights for us in understanding the role of host cuticle proteins during virus invasion. (C) 2017 Elsevier Ltd. All rights reserved.
关键词	Cuticle protein WSSV infection VP24 Litopenaeus vannamei
DOI	10.1016/j.dci.2017.12.018
收录类别	SCI
语种	英语
资助项目	NSFC-ISF Research Program of National Natural Science Foundation of China[31461143007] ; China Agriculture Research System-48[CARS-48] ; China Postdoctoral Science Foundation[2017M612361] ; NSFC-ISF Research Program of National Natural Science Foundation of China[31461143007] ; China Agriculture Research System-48[CARS-48] ; China Postdoctoral Science Foundation[2017M612361]
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000424726900031
出版者	ELSEVIER SCI LTD
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158064
专题	实验海洋生物学重点实验室
通讯作者	Li, Fuhua
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Yang, Feifei,Li, Shihao,Li, Fuhua,et al. A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,81:303-311.
APA	Yang, Feifei,Li, Shihao,Li, Fuhua,&Xiang, Jianhai.(2018).A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,81,303-311.
MLA	Yang, Feifei,et al."A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 81(2018):303-311.

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A cuticle protein fr（1614KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览