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A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection
Yang, Feifei1; Li, Shihao1; Li, Fuhua1,2; Xiang, Jianhai1,2
2018-04-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume81Pages:303-311
Corresponding AuthorLi, Fuhua(fhli@qdio.ac.cn)
AbstractWhite spot syndrome virus (WSSV) is a major viral pathogen in global shrimp farming, causing huge economic damage. Through penetrating the outer surface of the target tissues, WSSV enters into the cells of the target tissue to complete the replication process in the host. In the present study, a cuticle protein gene from Litopenaeus vannamei, designated as LvAMP13.4, was identified and proved to be involved in WSSV invasion. The deduced amino acid sequence of LvAMP13.4 contained a signal peptide and a conserved chitin-binding domain type 4 (ChBD4). This cuticle protein gene was mainly expressed in stomach, gill and epidermis. The expression level of LvAMP13.4 was significantly changed during WSSV infection. Silencing of LvAMP13.4 by dsRNA interference apparently reduced the mortality rate and the WSSV copy number in shrimp upon WSSV infection. Furthermore, yeast two-hybrid system and Co-IP assay were performed to confirm that LvAMP13.4 could interact with the major envelop protein VP24 of WSSV. These data indicated that LvAMP13.4 was involved in the invasion process of WSSV through interaction with VP24. The present results could provide new insights for us in understanding the role of host cuticle proteins during virus invasion. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordCuticle protein WSSV infection VP24 Litopenaeus vannamei
DOI10.1016/j.dci.2017.12.018
Indexed BySCI
Language英语
Funding ProjectNSFC-ISF Research Program of National Natural Science Foundation of China[31461143007] ; China Agriculture Research System-48[CARS-48] ; China Postdoctoral Science Foundation[2017M612361]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000424726900031
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158064
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Feifei,Li, Shihao,Li, Fuhua,et al. A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,81:303-311.
APA Yang, Feifei,Li, Shihao,Li, Fuhua,&Xiang, Jianhai.(2018).A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,81,303-311.
MLA Yang, Feifei,et al."A cuticle protein from the Pacific white shrimp Litopenaeus vannamei involved in WSSV infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 81(2018):303-311.
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