Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway | |
Yang, Yue1,2,3; Xing, Ronge1,2![]() ![]() ![]() | |
2018-03-01 | |
Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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ISSN | 0141-8130 |
Volume | 108Pages:1310-1321 |
Corresponding Author | Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
Abstract | To investigate the immunostimulatory effects of chitosan sulfates, we prepared alpha- and beta-chitosan sulfates with different molecular weights and compared their immunostimulatory activities in RAW 264.7 macrophages. Results suggest that beta-chitosan sulfates were more active than alpha-chitosan in promoting nitric oxide (NO) production. Further study show that beta-chitosan sulfate significantly promoted the production of NO, prostaglandin E-2, tumor necrosis factor (TNF)-alpha, interleukin-6 and interleukin-1 beta at the levels of transcription and translation. Moreover, Western blots revealed that it induced the phosphorylation of p85 and Akt, and the nuclear translocation of p50/p65 and c-Fos/c-Jun. The luciferase activity of cells pretreated with I3-chitosan sulfate further confirmed the nuclear translocation of p50/p65 and c-Fosic-Jun. Determination of Toll-like receptor (TLR) 4 expression suggested that beta-chitosan sulfate at least partly bound to TLR4. In conclusion, beta-chitosan sulfates activate RAW 264.7 cells through the PI3K-Akt pathway, which is dependent on activator protein-1 and nuclear factor-kappa B activation. (C) 2017 Elsevier B.V. All rights reserved. |
Keyword | Sulfated chitosan Immunostimulatory activity Nuclear factor-kappa B Toll like receptor-4 |
DOI | 10.1016/j.ijbiomac.2017.11.042 |
Indexed By | SCI |
Language | 英语 |
Funding Project | Qingdao science and technology plan[14-2-3-47-nsh] ; Shandong Provincial Science and Technology Major Project, China[2015ZDZX05003] ; Science and Technology Development Program of Shandong Province[2014GGH215006] ; commonweal item of State Oceanic Administration People's Republic of China[201405038-2] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao science and technology plan[14-2-3-47-nsh] ; Shandong Provincial Science and Technology Major Project, China[2015ZDZX05003] ; Science and Technology Development Program of Shandong Province[2014GGH215006] ; commonweal item of State Oceanic Administration People's Republic of China[201405038-2] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] |
WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
WOS ID | WOS:000425071500149 |
Publisher | ELSEVIER SCIENCE BV |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.qdio.ac.cn/handle/337002/158057 |
Collection | 实验海洋生物学重点实验室 |
Corresponding Author | Xing, Ronge; Li, Pengcheng |
Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
Recommended Citation GB/T 7714 | Yang, Yue,Xing, Ronge,Liu, Song,et al. Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,108:1310-1321. |
APA | Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2018).Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,108,1310-1321. |
MLA | Yang, Yue,et al."Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 108(2018):1310-1321. |
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