Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway | |
Yang, Yue1,2,3; Xing, Ronge1,2 ; Liu, Song1,2; Qin, Yukun1,2; Li, Kecheng2; Yu, Huahua1,2; Li, Pengcheng1,2
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| 2018-03-01 | |
| Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| Volume | 108Pages:1310-1321 |
| Corresponding Author | Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
| Abstract | To investigate the immunostimulatory effects of chitosan sulfates, we prepared alpha- and beta-chitosan sulfates with different molecular weights and compared their immunostimulatory activities in RAW 264.7 macrophages. Results suggest that beta-chitosan sulfates were more active than alpha-chitosan in promoting nitric oxide (NO) production. Further study show that beta-chitosan sulfate significantly promoted the production of NO, prostaglandin E-2, tumor necrosis factor (TNF)-alpha, interleukin-6 and interleukin-1 beta at the levels of transcription and translation. Moreover, Western blots revealed that it induced the phosphorylation of p85 and Akt, and the nuclear translocation of p50/p65 and c-Fos/c-Jun. The luciferase activity of cells pretreated with I3-chitosan sulfate further confirmed the nuclear translocation of p50/p65 and c-Fosic-Jun. Determination of Toll-like receptor (TLR) 4 expression suggested that beta-chitosan sulfate at least partly bound to TLR4. In conclusion, beta-chitosan sulfates activate RAW 264.7 cells through the PI3K-Akt pathway, which is dependent on activator protein-1 and nuclear factor-kappa B activation. (C) 2017 Elsevier B.V. All rights reserved. |
| Keyword | Sulfated chitosan Immunostimulatory activity Nuclear factor-kappa B Toll like receptor-4 |
| DOI | 10.1016/j.ijbiomac.2017.11.042 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Qingdao science and technology plan[14-2-3-47-nsh] ; Shandong Provincial Science and Technology Major Project, China[2015ZDZX05003] ; Science and Technology Development Program of Shandong Province[2014GGH215006] ; commonweal item of State Oceanic Administration People's Republic of China[201405038-2] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao science and technology plan[14-2-3-47-nsh] ; Shandong Provincial Science and Technology Major Project, China[2015ZDZX05003] ; Science and Technology Development Program of Shandong Province[2014GGH215006] ; commonweal item of State Oceanic Administration People's Republic of China[201405038-2] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] |
| WOS Research Area | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS Subject | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS ID | WOS:000425071500149 |
| Publisher | ELSEVIER SCIENCE BV |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/158057 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Xing, Ronge; Li, Pengcheng |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Yang, Yue,Xing, Ronge,Liu, Song,et al. Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,108:1310-1321. |
| APA | Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2018).Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,108,1310-1321. |
| MLA | Yang, Yue,et al."Immunostimulatory effects of sulfated chitosans on RAW 264.7 mouse macrophages via the activation of PI3 K/Akt signaling pathway".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 108(2018):1310-1321. |
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| Immunostimulatory ef（2800KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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