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Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit
Wu, Jing1,2,3; Chen, Huaxin1,2; Jiang, Peng1,2
2018
发表期刊BIOENGINEERED
ISSN2165-5979
卷号9期号:1页码:108-115
通讯作者Chen, Huaxin(chenhx001@126.com) ; Jiang, Peng(Jiangpeng@qdio.ac.cn)
摘要The fusion protein (SLA) of streptavidin and allophycocyanin subunit (holo-ApcA) was biosynthesized in Escherichia coli by a dual plasmid system. The recombinant SLA, purified by affinity chromatography, showed spectral properties similar to natural allophycocyanin subunit (ApcA). Spectral and Zinc staining analysis indicated that the recombinant SLA covalently bound phycocyanobilin (PCB). To improve chromophorylation rate of recombinant SLA, an in vitro chromophore attachment reaction system was established, which contained partially chromophylated SLA, PCB and lyase CpcS. Spectral analysis showed that PCB bound to the recombinant SLA rapidly during the reaction. The chromophorylation rate of SLA was improved from 21.1% to 86.5%. Immunofluorescence assay showed that SLA with high chromophorylation rate had higher detection signal. Thus, in vitro chromophore attachment is an effective way to improve the chromophorylation rate of recombinant phycobiliprotein.
关键词Allophycocyanin Chromophore attachment Chromophorylation rate Immunofluorescence assay Phycobiliprotein
DOI10.1080/21655979.2017.1321282
收录类别SCI
语种英语
资助项目National Natural Science Foundation[41276164] ; National 863 Program[2014AA093505] ; National 863 Program[2014AA093501] ; Development Funds of Science and Technology of Shinan District[2016-3-010-ZH] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Natural Science Foundation[41276164] ; National 863 Program[2014AA093505] ; National 863 Program[2014AA093501] ; development funds of science and technology of Shinan district[2016-3-010-ZH] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000425053900001
出版者TAYLOR & FRANCIS INC
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/158039
专题实验海洋生物学重点实验室
通讯作者Chen, Huaxin; Jiang, Peng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位实验海洋生物学重点实验室
通讯作者单位实验海洋生物学重点实验室
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GB/T 7714
Wu, Jing,Chen, Huaxin,Jiang, Peng. Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit[J]. BIOENGINEERED,2018,9(1):108-115.
APA Wu, Jing,Chen, Huaxin,&Jiang, Peng.(2018).Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit.BIOENGINEERED,9(1),108-115.
MLA Wu, Jing,et al."Chromophore attachment to fusion protein of streptavidin and recombinant allophycocyanin alpha subunit".BIOENGINEERED 9.1(2018):108-115.
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