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| The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities | |
| Jin, Weihua1,2; Liu, Bing1; Li, Shuai1; Chen, Jing1; Tang, Hong1; Jiang, Di1; Zhang, Quanbin2; Zhong, Weihong1 | |
| 2018-03-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| ISSN | 0141-8130 |
| 卷号 | 108页码:307-313 |
| 通讯作者 | Zhang, Quanbin(qbzhang@qdio.ac.cn) ; Zhong, Weihong(whzhong@zjut.edu.cn) |
| 摘要 | Polysaccharide (ST) was prepared from Sargassum thunbergii using hot water. Two fractions (ST-1 and ST-2) were prepared using anion exchange chromatography. One desulfated polysaccharide (ST-1-DS) was also prepared. Electrospray ionization mass spectrometry (ESI MS) performed on ST-1-DS showed that the desulfated polysaccharides contained methyl glycosides of mono-sulfated and di-sulfated galactofucooligosaccharides. This result suggested that ST-1 might contain sulfated galactofucan, which consists of a backbone of alternating (Gal) and (Fuc)(n) and sulfated randomly on Gal and mainly on C-2 in Fuc. In addition, ST-1 was degraded in 1 M sulfuric acid. The solution was centrifuged, and the supernatant was concentrated and precipitated in ethanol to obtain the precipitate (ST-1-P). ST-1-P was then separated using gel chromatography and anion exchange chromatography to obtain the oligomers. ESI MS spectra of oligomers indicated that ST-1 mostly contained sulfated glucuronomannan and fucoglucuronan. ESI MS with collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) suggested that glucuronomannan contained alternating 2-linked Man and 4-linked GlcA, while fucoglucuronan contained 4-linked glucuronan with branched Fuc at C-3. Finally, the neuroprotective activities of ST, ST-1, ST-2 and MIX (a mixture of ST-1 and ST-2) were determined. ST showed the most neuroprotective activity, which indicated that ST might be a good candidate for curing neurodegenerative diseases. (C) 2017 Elsevier B.V. All rights reserved. |
| 关键词 | Heteropolysaccharide Structural analysis Sargassum thunbergii |
| DOI | 10.1016/j.ijbiomac.2017.12.009 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Natural Science Foundation of China[41506165] ; Science and Technology program of applied basic research projects of Qingdao Municipal[15-9-1-74-jch] ; Shandong Provincial Natural Science Foundation, China[ZR2014DQ024] ; National Natural Science Foundation of China[41506165] ; Science and Technology program of applied basic research projects of Qingdao Municipal[15-9-1-74-jch] ; Shandong Provincial Natural Science Foundation, China[ZR2014DQ024] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| WOS记录号 | WOS:000425071500037 |
| 出版者 | ELSEVIER SCIENCE BV |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/158020 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Quanbin; Zhong, Weihong |
| 作者单位 | 1.Zhejiang Univ Technol, Coll Biotechnol & Bioengineering, Hangzhou 310014, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Jin, Weihua,Liu, Bing,Li, Shuai,et al. The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,108:307-313. |
| APA | Jin, Weihua.,Liu, Bing.,Li, Shuai.,Chen, Jing.,Tang, Hong.,...&Zhong, Weihong.(2018).The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,108,307-313. |
| MLA | Jin, Weihua,et al."The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 108(2018):307-313. |
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