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The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities
Jin, Weihua1,2; Liu, Bing1; Li, Shuai1; Chen, Jing1; Tang, Hong1; Jiang, Di1; Zhang, Quanbin2; Zhong, Weihong1
2018-03-01
Source PublicationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
Volume108Pages:307-313
Corresponding AuthorZhang, Quanbin(qbzhang@qdio.ac.cn) ; Zhong, Weihong(whzhong@zjut.edu.cn)
AbstractPolysaccharide (ST) was prepared from Sargassum thunbergii using hot water. Two fractions (ST-1 and ST-2) were prepared using anion exchange chromatography. One desulfated polysaccharide (ST-1-DS) was also prepared. Electrospray ionization mass spectrometry (ESI MS) performed on ST-1-DS showed that the desulfated polysaccharides contained methyl glycosides of mono-sulfated and di-sulfated galactofucooligosaccharides. This result suggested that ST-1 might contain sulfated galactofucan, which consists of a backbone of alternating (Gal) and (Fuc)(n) and sulfated randomly on Gal and mainly on C-2 in Fuc. In addition, ST-1 was degraded in 1 M sulfuric acid. The solution was centrifuged, and the supernatant was concentrated and precipitated in ethanol to obtain the precipitate (ST-1-P). ST-1-P was then separated using gel chromatography and anion exchange chromatography to obtain the oligomers. ESI MS spectra of oligomers indicated that ST-1 mostly contained sulfated glucuronomannan and fucoglucuronan. ESI MS with collision-induced dissociation tandem mass spectrometry (ESI-CID-MS/MS) suggested that glucuronomannan contained alternating 2-linked Man and 4-linked GlcA, while fucoglucuronan contained 4-linked glucuronan with branched Fuc at C-3. Finally, the neuroprotective activities of ST, ST-1, ST-2 and MIX (a mixture of ST-1 and ST-2) were determined. ST showed the most neuroprotective activity, which indicated that ST might be a good candidate for curing neurodegenerative diseases. (C) 2017 Elsevier B.V. All rights reserved.
KeywordHeteropolysaccharide Structural analysis Sargassum thunbergii
DOI10.1016/j.ijbiomac.2017.12.009
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41506165] ; Science and Technology program of applied basic research projects of Qingdao Municipal[15-9-1-74-jch] ; Shandong Provincial Natural Science Foundation, China[ZR2014DQ024]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS IDWOS:000425071500037
PublisherELSEVIER SCIENCE BV
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158020
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Quanbin; Zhong, Weihong
Affiliation1.Zhejiang Univ Technol, Coll Biotechnol & Bioengineering, Hangzhou 310014, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
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GB/T 7714
Jin, Weihua,Liu, Bing,Li, Shuai,et al. The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,108:307-313.
APA Jin, Weihua.,Liu, Bing.,Li, Shuai.,Chen, Jing.,Tang, Hong.,...&Zhong, Weihong.(2018).The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,108,307-313.
MLA Jin, Weihua,et al."The structural features of the sulfated heteropolysaccharide (ST-1) from Sargassum thunbergii and its neuroprotective activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 108(2018):307-313.
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