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A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri
Wang, Mengqiang1; Wang, Baojie1; Jiang, Keyong1; Liu, Mei1; Shi, Xiaowei1,3,4; Wang, Lei1,2
2018
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号72页码:282-290
通讯作者Wang, Lei(wanglei@qdio.ac.cn)
摘要Superoxide dismutase (SOD) ubiquitously found in both prokaryotes and eukaryotes functions as the first and essential enzyme in the antioxidant system. In the present study, a manganese SOD (designated as CfmtMnSOD) was cloned from Zhikong scallop Chlamys farreri. The complete cDNA sequence of CfmtMnSOD contained a 681 bp open reading frame (ORF), encoding a peptide of 226 amino acids. A SOD_Fe_N domain and a SOD_Fe_C domain were found in the deduced amino acid sequence of CfmtMnSOD. The mRNA transcripts of CfmtMnSOD were constitutively expressed in all the tested tissues, including gill, gonad, hepatopancreas, hemocytes, mantle and muscle, with the highest expression level in hemocytes. After the stimulation of Vibrio splendidus, Staphylococcus aureus and Yarrowia lipolytica, the mRNA transcripts of CfmtMnSOD in hemocytes all significantly increased. The purified rCfmtMnSOD protein exhibited Mn2+ dependent specific and low stable enzymatic activities. After Vibrio challenge, the cumulative mortality of CfmtMnSOD-suppressed scallops was significantly higher than those of control groups and the semi-lethal time for CfmtMnSOD-suppressed scallops was rather shorter than those of control groups either. Moreover, the final mortality rate of CfmtMnSOD-suppressed group was significant higher than those of control groups, even without Vibrio challenge. All these results indicated that CfmtMnSOD was efficient antioxidant enzyme involved in the innate immunity, and also essential for the survival of C. farreri.
关键词Chlamys farreri Innate immunity Manganese superoxide dismutase
DOI10.1016/j.fsi.2017.11.010
收录类别SCI
语种英语
资助项目Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002] ; China Postdoctoral Science Foundation[2017M610427] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002] ; China Postdoctoral Science Foundation[2017M610427]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号WOS:000423644600033
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计
被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/158005
专题实验海洋生物学重点实验室
通讯作者Wang, Lei
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Linyi Univ, Linyi 276000, Peoples R China
4.Shandong Prov Engn Technol Res Ctr Liman Chinese, Linyi 276000, Peoples R China
第一作者单位实验海洋生物学重点实验室
通讯作者单位实验海洋生物学重点实验室
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Wang, Mengqiang,Wang, Baojie,Jiang, Keyong,et al. A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:282-290.
APA Wang, Mengqiang,Wang, Baojie,Jiang, Keyong,Liu, Mei,Shi, Xiaowei,&Wang, Lei.(2018).A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,72,282-290.
MLA Wang, Mengqiang,et al."A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 72(2018):282-290.
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