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A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri
Wang, Mengqiang1; Wang, Baojie1; Jiang, Keyong1; Liu, Mei1; Shi, Xiaowei1,3,4; Wang, Lei1,2
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume72Pages:282-290
Corresponding AuthorWang, Lei(wanglei@qdio.ac.cn)
AbstractSuperoxide dismutase (SOD) ubiquitously found in both prokaryotes and eukaryotes functions as the first and essential enzyme in the antioxidant system. In the present study, a manganese SOD (designated as CfmtMnSOD) was cloned from Zhikong scallop Chlamys farreri. The complete cDNA sequence of CfmtMnSOD contained a 681 bp open reading frame (ORF), encoding a peptide of 226 amino acids. A SOD_Fe_N domain and a SOD_Fe_C domain were found in the deduced amino acid sequence of CfmtMnSOD. The mRNA transcripts of CfmtMnSOD were constitutively expressed in all the tested tissues, including gill, gonad, hepatopancreas, hemocytes, mantle and muscle, with the highest expression level in hemocytes. After the stimulation of Vibrio splendidus, Staphylococcus aureus and Yarrowia lipolytica, the mRNA transcripts of CfmtMnSOD in hemocytes all significantly increased. The purified rCfmtMnSOD protein exhibited Mn2+ dependent specific and low stable enzymatic activities. After Vibrio challenge, the cumulative mortality of CfmtMnSOD-suppressed scallops was significantly higher than those of control groups and the semi-lethal time for CfmtMnSOD-suppressed scallops was rather shorter than those of control groups either. Moreover, the final mortality rate of CfmtMnSOD-suppressed group was significant higher than those of control groups, even without Vibrio challenge. All these results indicated that CfmtMnSOD was efficient antioxidant enzyme involved in the innate immunity, and also essential for the survival of C. farreri.
KeywordChlamys farreri Innate immunity Manganese superoxide dismutase
DOI10.1016/j.fsi.2017.11.010
Indexed BySCI
Language英语
Funding ProjectKey Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002] ; China Postdoctoral Science Foundation[2017M610427]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000423644600033
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158005
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Linyi Univ, Linyi 276000, Peoples R China
4.Shandong Prov Engn Technol Res Ctr Liman Chinese, Linyi 276000, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Mengqiang,Wang, Baojie,Jiang, Keyong,et al. A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:282-290.
APA Wang, Mengqiang,Wang, Baojie,Jiang, Keyong,Liu, Mei,Shi, Xiaowei,&Wang, Lei.(2018).A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,72,282-290.
MLA Wang, Mengqiang,et al."A mitochondrial manganese superoxide dismutase involved in innate immunity is essential for the survival of Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 72(2018):282-290.
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