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Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians
Wang, M.1,3; Wang, B.1; Liu, M.1; Jiang, K.1; Wang, L.1,2
2018
发表期刊ISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN1824-307X
卷号15页码:19-30
通讯作者Wang, L.(wanglei@qdio.ac.cn)
摘要Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics and also play important roles in innate immunity. In the present study, a novel sigma class GST gene (designated as AiGST sigma) was cloned from the bay scallop Argopecten irradians via rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of AiGSTs consisted of a 5' untranslated regions (UTR) of 48 bp, a 3' UTR of 113 bp with a poly A tail and an open reading frame (ORF) of 618 bp. The ORF encoded a polypeptide of 205 amino acid residues with a calculated molecular mass of approximately 23.11 kDa and a theoretical isoelectric point of 5.354. The deduced amino acid sequence of AiGSTs contained a GST_N domain and a GST_C domain, and exhibited high similarity with other reported sigma class GSTs. In the phylogenetic tree, AiGSTs was located in the sigma class GSTs sub-branch. The AiGSTs mRNA transcripts were constitutively expressed in the tissues of hemocytes, muscle, mantle, gill, hepatopancreas and gonad, with the highest expression level in hemocytes, and the mRNA expression levels of AiGSTs were significantly up-regulated in hemocytes after various pathogen associated molecular patterns (PAMPs) stimulation. The purified recombinant AiGSTs protein exhibited catalytic activity against the common substrate 1-chloro-2, 4-dinitrobenzene (CDNB) with low thermal stability and narrow optimum pH spectrum. All these results indicated that AiGSTs was a fragile but efficient antioxidant enzyme and was potentially involved in the innate immune responses of scallop.
关键词Argopecten irradians glutathione S-transferase innate immunity
收录类别SCI
语种英语
资助项目Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106]
WOS研究方向Immunology ; Zoology
WOS类目Immunology ; Zoology
WOS记录号WOS:000423724700004
出版者INVERTEBRATE SURVIVAL JOURNAL
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/157993
专题实验海洋生物学重点实验室
通讯作者Wang, L.
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China
第一作者单位实验海洋生物学重点实验室
通讯作者单位实验海洋生物学重点实验室
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Wang, M.,Wang, B.,Liu, M.,et al. Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:19-30.
APA Wang, M.,Wang, B.,Liu, M.,Jiang, K.,&Wang, L..(2018).Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,19-30.
MLA Wang, M.,et al."Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):19-30.
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