Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei | |
Barreto, Caire1; Coelho, Jaqueline da Rosa1; Yuan, Jianbo2; Xiang, Jianhai2![]() | |
2018 | |
Source Publication | MARINE DRUGS
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ISSN | 1660-3397 |
Volume | 16Issue:1Pages:16 |
Corresponding Author | Rosa, Rafael Diego(rafael.d.rosa@ufsc.br) |
Abstract | Crustins form a large family of antimicrobial peptides (AMPs) in crustaceans composed of four sub-groups (Types I-IV). Type II crustins (Type IIa or Crustins and Type IIb or Crustin-like) possess a typical hydrophobic N-terminal region and are by far the most representative sub-group found in penaeid shrimp. To gain insight into the molecular diversity of Type II crustins in penaeids, we identified and characterized a Type IIb crustin in Litopenaeus vannamei (Crustin-like Lv) and compared Type II crustins at both molecular and transcriptional levels. Although L. vannamei Type II crustins (Crustin Lv and Crustin-like Lv) are encoded by separate genes, they showed a similar tissue distribution (hemocytes and gills) and transcriptional response to the shrimp pathogens Vibrio harveyi and White spot syndrome virus (WSSV). As Crustin Lv, Crustin-like Lv transcripts were found to be present early in development, suggesting a maternal contribution to shrimp progeny. Altogether, our in silico and transcriptional data allowed to conclude that (1) each sub-type displays a specific amino acid signature at the C-terminal end holding both the cysteine-rich region and the whey acidic protein (WAP) domain, and that (2) shrimp Type II crustins evolved from a common ancestral gene that conserved a similar pattern of transcriptional regulation. |
Keyword | invertebrate immunity host defense peptide crustacean WAP domain molecular diversity host-pathogen interaction |
DOI | 10.3390/md16010031 |
Indexed By | SCI |
Language | 英语 |
Funding Project | CNPq[MEC/MCTI/CAPES/CNPq/FAPs PVE 401191/2014-1] ; CNPq[MCTI/CNPq Universal 406530/2016-5] ; CAPES[CIMAR 1974/2014] ; National High-Technology Research and Development Program of China (863 program)[2012AA10A404] ; National High-Technology Research and Development Program of China (863 program)[2012AA092205] ; CAPES ; CNPq[MEC/MCTI/CAPES/CNPq/FAPs PVE 401191/2014-1] ; CNPq[MCTI/CNPq Universal 406530/2016-5] ; CAPES[CIMAR 1974/2014] ; National High-Technology Research and Development Program of China (863 program)[2012AA10A404] ; National High-Technology Research and Development Program of China (863 program)[2012AA092205] ; CAPES |
WOS Research Area | Pharmacology & Pharmacy |
WOS Subject | Chemistry, Medicinal |
WOS ID | WOS:000424125600031 |
Publisher | MDPI AG |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.qdio.ac.cn/handle/337002/157836 |
Collection | 实验海洋生物学重点实验室 |
Corresponding Author | Rosa, Rafael Diego |
Affiliation | 1.Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Lab Immunol Appl Aquaculture, BR-88040900 Florianopolis, SC, Brazil 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
Recommended Citation GB/T 7714 | Barreto, Caire,Coelho, Jaqueline da Rosa,Yuan, Jianbo,et al. Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei[J]. MARINE DRUGS,2018,16(1):16. |
APA | Barreto, Caire,Coelho, Jaqueline da Rosa,Yuan, Jianbo,Xiang, Jianhai,Perazzolo, Luciane Maria,&Rosa, Rafael Diego.(2018).Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei.MARINE DRUGS,16(1),16. |
MLA | Barreto, Caire,et al."Specific Molecular Signatures for Type II Crustins in Penaeid Shrimp Uncovered by the Identification of Crustin-Like Antimicrobial Peptides in Litopenaeus vannamei".MARINE DRUGS 16.1(2018):16. |
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