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A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda
Sun, Yuying1,4; Zhang, Jiquan2,3; Xiang, Jianhai2,3
2017-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume71Pages:43-49
Corresponding AuthorZhang, Jiquan(zhangjiquan@qdio.ac.cn)
AbstractChitinase, belonging to family 18 glycosyl hydrolase, is a multi-gene family and it has many functions. Generation of loss-of-function mutant targeting an interesting gene is a common way to clarify its function based on reverse genetics. In this study, we first reported the immune defense of a chitinase gene (EcChi4) in Exopalaemon carinicauda using its EcChi4-deletion mutant. EcChi4 was predominantly expressed in hepatopancreas and was upregulated after challenge with Vibrio parahaemolyticus or Aeromonas hydrophila. After knockout EcChi4 gene using CRISPR/Cas9 tool, the prawns in EcChi4-deletion group had significant higher mortality than those in wild-type group when the prawns were challenged with V. parahaemolyticus or A. hydrophila. In conclusion, we first demonstrate the function of a chitinase gene in immune defense of E. carinicauda by performing directed, heritable gene mutagenesis. In the future, CRISPR/Cas9 should be widely applicable as a feasible means for gene editing in E. carinicauda for the study of important biological questions that cannot be easily addressed in other decapods. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordExopalaemon carinicauda Chitinase CRISPR/Cas9 Hepatopancreas
DOI10.1016/j.fsi.2017.09.065
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31172449] ; National Natural Science Foundation of China[41376165] ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; National High Technology Research and Development Program of China[2012AA10A401]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000418309100006
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/157790
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Jiquan
Affiliation1.Huaihai Inst Technol, Coll Marine Life & Fisheries, 59 Cangwu Rd, Lianyungang 222005, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
4.Huaihai Inst Technol, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Yuying,Zhang, Jiquan,Xiang, Jianhai. A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda[J]. FISH & SHELLFISH IMMUNOLOGY,2017,71:43-49.
APA Sun, Yuying,Zhang, Jiquan,&Xiang, Jianhai.(2017).A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda.FISH & SHELLFISH IMMUNOLOGY,71,43-49.
MLA Sun, Yuying,et al."A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda".FISH & SHELLFISH IMMUNOLOGY 71(2017):43-49.
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