A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda

doi:10.1016/j.fsi.2017.09.065

IOCAS-IR > 实验海洋生物学重点实验室

	A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda
	Sun, Yuying 1,4; Zhang, Jiquan 2,3; Xiang, Jianhai2,3
	2017-12-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	71 页码:43-49
通讯作者	Zhang, Jiquan(zhangjiquan@qdio.ac.cn)
摘要	Chitinase, belonging to family 18 glycosyl hydrolase, is a multi-gene family and it has many functions. Generation of loss-of-function mutant targeting an interesting gene is a common way to clarify its function based on reverse genetics. In this study, we first reported the immune defense of a chitinase gene (EcChi4) in Exopalaemon carinicauda using its EcChi4-deletion mutant. EcChi4 was predominantly expressed in hepatopancreas and was upregulated after challenge with Vibrio parahaemolyticus or Aeromonas hydrophila. After knockout EcChi4 gene using CRISPR/Cas9 tool, the prawns in EcChi4-deletion group had significant higher mortality than those in wild-type group when the prawns were challenged with V. parahaemolyticus or A. hydrophila. In conclusion, we first demonstrate the function of a chitinase gene in immune defense of E. carinicauda by performing directed, heritable gene mutagenesis. In the future, CRISPR/Cas9 should be widely applicable as a feasible means for gene editing in E. carinicauda for the study of important biological questions that cannot be easily addressed in other decapods. (C) 2017 Elsevier Ltd. All rights reserved.
关键词	Exopalaemon carinicauda Chitinase CRISPR/Cas9 Hepatopancreas
DOI	10.1016/j.fsi.2017.09.065
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31172449] ; National Natural Science Foundation of China[41376165] ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; National High Technology Research and Development Program of China[2012AA10A401] ; National Natural Science Foundation of China[31172449] ; National Natural Science Foundation of China[41376165] ; Priority Academic Program Development of Jiangsu Higher Education Institutions ; National High Technology Research and Development Program of China[2012AA10A401]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000418309100006
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：21[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157790
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Jiquan
作者单位	1.Huaihai Inst Technol, Coll Marine Life & Fisheries, 59 Cangwu Rd, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 4.Huaihai Inst Technol, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Sun, Yuying,Zhang, Jiquan,Xiang, Jianhai. A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda[J]. FISH & SHELLFISH IMMUNOLOGY,2017,71:43-49.
APA	Sun, Yuying,Zhang, Jiquan,&Xiang, Jianhai.(2017).A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda.FISH & SHELLFISH IMMUNOLOGY,71,43-49.
MLA	Sun, Yuying,et al."A CRISPR/Cas9-mediated mutation in chitinase changes immune response to bacteria in Exopalaemon carinicauda".FISH & SHELLFISH IMMUNOLOGY 71(2017):43-49.