Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid

doi:10.3389/fmicb.2017.02450

	Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid
	Deng, Yunyan 1,2; Hu, Zhangxi1,2 ; Shang, Lixia 1; Peng, Quancai 3; Tang, Ying Zhong 1,2
	2017-12-11
发表期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
卷号	8 页码:19
通讯作者	Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
摘要	Due to the vital importance of resting cysts in the biology and ecology of many dinoflagellates, a transcriptomic investigation on Scrippsiella trochoidea was conducted with the aim to reveal the molecular processes and relevant functional genes regulating encystment and dormancy in dinoflagellates. We identified via RNA-seq 3,874 (out of 166,575) differentially expressed genes (DEGs) between resting cysts and vegetative cells; a pause of photosynthesis (confirmed via direct measurement of photosynthetic efficiency); an active catabolism including beta-oxidation, glycolysis, glyoxylate pathway, and TCA in resting cysts (tested via measurements of respiration rate); 12 DEGs encoding meiotic recombination proteins and members of MEI2-like family potentially involved in sexual reproduction and encystment; elevated expressions in genes encoding enzymes responding to pathogens (chitin deacetylase) and ROS stress in cysts; and 134 unigenes specifically expressed in cysts. We paid particular attention to genes pertaining to phytohormone signaling and identified 4 key genes regulating abscisic acid (ABA) biosynthesis and catabolism, with further characterization based on their full-length cDNA obtained via RACE-PCR. The qPCR results demonstrated elevated biosynthesis and repressed catabolism of ABA during the courses of encystment and cyst dormancy, which was significantly enhanced by lower temperature (4 +/- 1 degrees C) and darkness. Direct measurements of ABA using UHPLC-MS/MS and ELISA in vegetative cells and cysts both fully supported qPCR results. These results collectively suggest a vital role of ABA in regulating encystment and maintenance of dormancy, akin to its function in seed dormancy of higher plants. Our results provided a critical advancement in understanding molecular processes in resting cysts of dinoflagellates.
关键词	resting cyst harmful algal blooms (HABs) dormancy UHPLC-MS/MS abscisicacid (ABA) dinoflagellate Scrippsiella trochoidea
DOI	10.3389/fmicb.2017.02450
收录类别	SCI
语种	英语
资助项目	NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; National Science Foundation of China[41476142] ; National Science Foundation of China[41606126] ; National Science Foundation of China[61533011] ; National Science Foundation of China[41506143] ; National Science Foundation of China[U1301235] ; Scientific and Technological Innovation Project ; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; National Science Foundation of China[41476142] ; National Science Foundation of China[41606126] ; National Science Foundation of China[61533011] ; National Science Foundation of China[41506143] ; National Science Foundation of China[U1301235] ; Scientific and Technological Innovation Project ; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000417577500001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：31[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157781
专题	海洋生态与环境科学重点实验室所级公共技术中心
通讯作者	Tang, Ying Zhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Res Ctr Anal & Measurement, Qingdao, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Deng, Yunyan,Hu, Zhangxi,Shang, Lixia,et al. Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid[J]. FRONTIERS IN MICROBIOLOGY,2017,8:19.
APA	Deng, Yunyan,Hu, Zhangxi,Shang, Lixia,Peng, Quancai,&Tang, Ying Zhong.(2017).Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid.FRONTIERS IN MICROBIOLOGY,8,19.
MLA	Deng, Yunyan,et al."Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid".FRONTIERS IN MICROBIOLOGY 8(2017):19.