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Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid
Deng, Yunyan1,2; Hu, Zhangxi1,2; Shang, Lixia1; Peng, Quancai3; Tang, Ying Zhong1,2
2017-12-11
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume8Pages:19
Corresponding AuthorTang, Ying Zhong(yingzhong.tang@qdio.ac.cn)
AbstractDue to the vital importance of resting cysts in the biology and ecology of many dinoflagellates, a transcriptomic investigation on Scrippsiella trochoidea was conducted with the aim to reveal the molecular processes and relevant functional genes regulating encystment and dormancy in dinoflagellates. We identified via RNA-seq 3,874 (out of 166,575) differentially expressed genes (DEGs) between resting cysts and vegetative cells; a pause of photosynthesis (confirmed via direct measurement of photosynthetic efficiency); an active catabolism including beta-oxidation, glycolysis, glyoxylate pathway, and TCA in resting cysts (tested via measurements of respiration rate); 12 DEGs encoding meiotic recombination proteins and members of MEI2-like family potentially involved in sexual reproduction and encystment; elevated expressions in genes encoding enzymes responding to pathogens (chitin deacetylase) and ROS stress in cysts; and 134 unigenes specifically expressed in cysts. We paid particular attention to genes pertaining to phytohormone signaling and identified 4 key genes regulating abscisic acid (ABA) biosynthesis and catabolism, with further characterization based on their full-length cDNA obtained via RACE-PCR. The qPCR results demonstrated elevated biosynthesis and repressed catabolism of ABA during the courses of encystment and cyst dormancy, which was significantly enhanced by lower temperature (4 +/- 1 degrees C) and darkness. Direct measurements of ABA using UHPLC-MS/MS and ELISA in vegetative cells and cysts both fully supported qPCR results. These results collectively suggest a vital role of ABA in regulating encystment and maintenance of dormancy, akin to its function in seed dormancy of higher plants. Our results provided a critical advancement in understanding molecular processes in resting cysts of dinoflagellates.
Keywordresting cyst harmful algal blooms (HABs) dormancy UHPLC-MS/MS abscisicacid (ABA) dinoflagellate Scrippsiella trochoidea
DOI10.3389/fmicb.2017.02450
Indexed BySCI
Language英语
Funding ProjectNSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; National Science Foundation of China[41476142] ; National Science Foundation of China[41606126] ; National Science Foundation of China[61533011] ; National Science Foundation of China[41506143] ; National Science Foundation of China[U1301235] ; Scientific and Technological Innovation Project ; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02]
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000417577500001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/157781
Collection海洋生态与环境科学重点实验室
分析测试中心
Corresponding AuthorTang, Ying Zhong
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Res Ctr Anal & Measurement, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Deng, Yunyan,Hu, Zhangxi,Shang, Lixia,et al. Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid[J]. FRONTIERS IN MICROBIOLOGY,2017,8:19.
APA Deng, Yunyan,Hu, Zhangxi,Shang, Lixia,Peng, Quancai,&Tang, Ying Zhong.(2017).Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid.FRONTIERS IN MICROBIOLOGY,8,19.
MLA Deng, Yunyan,et al."Transcriptomic Analyses of Scrippsiella trochoidea Reveals Processes Regulating Encystment and Dormancy in the Life Cycle of a Dinoflagellate, with a Particular Attention to the Role of Abscisic Acid".FRONTIERS IN MICROBIOLOGY 8(2017):19.
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