Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites

doi:10.1016/j.fsi.2018.09.011

	Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites
	Li, Meng1 ; Huang, Qian 1,2; Wang, Jinfeng1,2 ; Li, Caiwen 1,3
	2018-12-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	83 页码:134-139
文章类型	Article
摘要	Latest studies have indicated that microRNAs (miRNAs) play important roles in defending against bacterial and viral infections in marine crustacean, whereas little is known regarding the immunological roles of crustacean miRNAs in response to parasitic infection. To further reveal the host-parasite interactions between the parasitic dinoflagellate Hematodinium and its crustacean hosts, we applied the high-throughput sequencing technology to identify and characterize miRNAs in the Chinese swimming crab Portunus trituberculatus challenged with the Hematodinium parasite at a timescale of 16 days (d). A total of 168 miRNAs were identified and 51 miRNAs were differentially expressed in the hepatopancreas tissues of affected hosts. Eleven of the differentially expressed miRNAs were selected and verified by the quantitative real-time RT-PCR (qRT-PCR), manifesting the consistency between the high throughout sequencing and qRT-PCR assays. Further analysis of the putative target genes indicated that various immune-related pathways (e.g. endocytosis, Fc gamma R-mediated phagocytosis, lysosome, ECM-receptor interaction, complement and coagulation cascades, antigen processing and presentation, focal adhesion, etc.) and signal transduction pathways (e.g. JAK-STAT signaling pathway, MAPK signaling pathway, p53 signaling pathway, etc.) were mediated by the differentially expressed miRNAs. The results presented fundamental knowledge on the immunological roles of crustacean miRNAs and contributed to the better understanding of hosts' miRNAs-mediated immunity against the parasitic infection.
关键词	Marine crustacean Parasite Innate immunity microRNA Host-parasite interaction
学科领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2018.09.011
收录类别	SCIE
语种	英语
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000448096200015
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157516
专题	海洋生态与环境科学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	海洋生态与环境科学重点实验室
推荐引用方式 GB/T 7714	Li, Meng,Huang, Qian,Wang, Jinfeng,et al. Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites[J]. FISH & SHELLFISH IMMUNOLOGY,2018,83:134-139.
APA	Li, Meng,Huang, Qian,Wang, Jinfeng,&Li, Caiwen.(2018).Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites.FISH & SHELLFISH IMMUNOLOGY,83,134-139.
MLA	Li, Meng,et al."Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites".FISH & SHELLFISH IMMUNOLOGY 83(2018):134-139.

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