Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites | |
Li, Meng1 ; Huang, Qian1,2; Wang, Jinfeng1,2; Li, Caiwen1,3
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| 2018-12-01 | |
| Source Publication | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| Volume | 83Pages:134-139 |
| Subtype | Article |
| Abstract | Latest studies have indicated that microRNAs (miRNAs) play important roles in defending against bacterial and viral infections in marine crustacean, whereas little is known regarding the immunological roles of crustacean miRNAs in response to parasitic infection. To further reveal the host-parasite interactions between the parasitic dinoflagellate Hematodinium and its crustacean hosts, we applied the high-throughput sequencing technology to identify and characterize miRNAs in the Chinese swimming crab Portunus trituberculatus challenged with the Hematodinium parasite at a timescale of 16 days (d). A total of 168 miRNAs were identified and 51 miRNAs were differentially expressed in the hepatopancreas tissues of affected hosts. Eleven of the differentially expressed miRNAs were selected and verified by the quantitative real-time RT-PCR (qRT-PCR), manifesting the consistency between the high throughout sequencing and qRT-PCR assays. Further analysis of the putative target genes indicated that various immune-related pathways (e.g. endocytosis, Fc gamma R-mediated phagocytosis, lysosome, ECM-receptor interaction, complement and coagulation cascades, antigen processing and presentation, focal adhesion, etc.) and signal transduction pathways (e.g. JAK-STAT signaling pathway, MAPK signaling pathway, p53 signaling pathway, etc.) were mediated by the differentially expressed miRNAs. The results presented fundamental knowledge on the immunological roles of crustacean miRNAs and contributed to the better understanding of hosts' miRNAs-mediated immunity against the parasitic infection. |
| Keyword | Marine crustacean Parasite Innate immunity microRNA Host-parasite interaction |
| Subject Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| DOI | 10.1016/j.fsi.2018.09.011 |
| Indexed By | SCIE |
| Language | 英语 |
| WOS Research Area | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS ID | WOS:000448096200015 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/157516 |
| Collection | 海洋生态与环境科学重点实验室 |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| First Author Affilication | Key Laboratory of Marine Ecology & Environmental Sciences, CAS |
| Recommended Citation GB/T 7714 | Li, Meng,Huang, Qian,Wang, Jinfeng,et al. Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites[J]. FISH & SHELLFISH IMMUNOLOGY,2018,83:134-139. |
| APA | Li, Meng,Huang, Qian,Wang, Jinfeng,&Li, Caiwen.(2018).Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites.FISH & SHELLFISH IMMUNOLOGY,83,134-139. |
| MLA | Li, Meng,et al."Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites".FISH & SHELLFISH IMMUNOLOGY 83(2018):134-139. |
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| File Name/Size | DocType | Version | Access | License | ||
| Differential express（1505KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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