Delineating the Origins of Vibrio parahaemolyticus Isolated from Outbreaks of Acute Hepatopancreatic Necrosis Disease in Asia by the Use of Whole Genome Sequencing

doi:10.3389/fmicb.2017.02354

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	Delineating the Origins of Vibrio parahaemolyticus Isolated from Outbreaks of Acute Hepatopancreatic Necrosis Disease in Asia by the Use of Whole Genome Sequencing
	Fu, Songzhe 1,2; Tian, Huiqin 3; Wei, Dawei 4; Zhang, Xiaojun 5; Liu, Ying1,6
	2017-11-28
发表期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
卷号	8 页码:12
通讯作者	Fu, Songzhe(fusongzhe@hotmail.com)
摘要	Acute hepatopancreatic necrosis disease (AHPND) is an emerging penaeid shrimp disease caused by Vibrio parahaemolyticus. Although V. parahaemolyticus has been isolated and sequenced from several Asia countries, the epidemiological links among the AHPND outbreaks in different locations remain unclear. In this study, we sequenced the genomes of nine strains isolated in China between 2014 and 2016 from four sampling sites in three provinces. Analysis of single nucleotide polymorphisms (SNPs) among the nine isolates yielded an average of 35,519 SNPs per isolate, ranging from 35,001 SNPs to 35,889 SNPs relative to the reference genome FDA_R31. To capture the genetic diversity of V. parahaemolyticus in Asia and Mexico, 93 published genomes were included in the analysis. Phylogenetic analysis divided the 102 isolates into 5 clades from I to V, with the majority belonging to Clade I and Clade II. There were at least 12 independent AHPND related clones. The results indicated that the clones recovered from AHPND affected shrimps in Asia were genomically distinct in various locations and there are no epidemiological links between Asian and Mexico outbreaks. Core genome analysis of pVA-1-like plasmid sequences from V. parahaemolyticus revealed that the AHPND-associated plasmids were also genetically diverse. Homology analysis of the publicly available microbial genomes showed that the conjugative transfer gene clusters of the plasmids in AHPND-causing strains were found in 27 V. parahaemolyticus strains and several other Vibrio sp. from 10 countries including five strains isolated prior to the first identification of AHPND outbreak, indicating that the backbone of AHPND-associated plasmid was widely distributed around the globe. In conclusion, at least 11 origins of AHPND outbreaks were identified; as AHPND-causing plasmid is widely distributed globally, prevention strategies for AHPND need to focus on microbial management in the aquaculture system and establishing ecological friendly aquaculture practices instead of detection of plasmid alone. However, more strains from other Asia countries as well as Mexico need to be included for whole genome sequencing (WGS) for reconstruction of the global transmission and the spread patterns of AHPND.
关键词	AHPND Vibrio parahaemolyticus SNPs whole genome sequencing pVA-1 like plasmid
DOI	10.3389/fmicb.2017.02354
收录类别	SCI
语种	英语
资助项目	National Natural Science Fund of China[31672673] ; National Natural Science Fund of China[31472312] ; National Natural Science Fund of China[31760492] ; Jiangsu Key RD Project[BE2015325] ; National Key R&D Program of China[2017YFD0701700] ; National Natural Science Fund of China[31672673] ; National Natural Science Fund of China[31472312] ; National Natural Science Fund of China[31760492] ; Jiangsu Key RD Project[BE2015325] ; National Key R&D Program of China[2017YFD0701700] ; National Natural Science Fund of China[31672673] ; National Natural Science Fund of China[31472312] ; National Natural Science Fund of China[31760492] ; Jiangsu Key RD Project[BE2015325] ; National key R&D Program of China[2017YFD0701700]
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:000416317200001
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157377
专题	中国科学院海洋研究所
通讯作者	Fu, Songzhe
作者单位	1.Dalian Ocean Univ, Coll Marine Technol & Environm, Dalian, Peoples R China 2.Nanchang Ctr Dis Control & Prevent, Nanchang, Jiangxi, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 4.Northwest Agr & Forestry Univ, Coll Life Sci, Yangling, Peoples R China 5.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China 6.Chinese Acad Sci, Nantong R&D Ctr, Nantong, Peoples R China
推荐引用方式 GB/T 7714	Fu, Songzhe,Tian, Huiqin,Wei, Dawei,et al. Delineating the Origins of Vibrio parahaemolyticus Isolated from Outbreaks of Acute Hepatopancreatic Necrosis Disease in Asia by the Use of Whole Genome Sequencing[J]. FRONTIERS IN MICROBIOLOGY,2017,8:12.
APA	Fu, Songzhe,Tian, Huiqin,Wei, Dawei,Zhang, Xiaojun,&Liu, Ying.(2017).Delineating the Origins of Vibrio parahaemolyticus Isolated from Outbreaks of Acute Hepatopancreatic Necrosis Disease in Asia by the Use of Whole Genome Sequencing.FRONTIERS IN MICROBIOLOGY,8,12.
MLA	Fu, Songzhe,et al."Delineating the Origins of Vibrio parahaemolyticus Isolated from Outbreaks of Acute Hepatopancreatic Necrosis Disease in Asia by the Use of Whole Genome Sequencing".FRONTIERS IN MICROBIOLOGY 8(2017):12.

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