Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)

doi:10.1534/g3.117.300210

	Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)
	Song, Hao 1,2,3; Qi, Lu 4; Zhang, Tao1,2 ; Wang, Hai-yan 1,2
	2017-12-01
发表期刊	G3-GENES GENOMES GENETICS
ISSN	2160-1836
卷号	7 期号:12 页码:3999-4008
通讯作者	Zhang, Tao(tzhang@qdio.ac.cn) ; Wang, Hai-yan(haiyanwang@qdio.ac.cn)
摘要	The veined rapa whelk (Rapana venosa) is widely consumed in China. Nevertheless, it preys on oceanic bivalves, thereby reducing this resource worldwide. Its larval metamorphosis comprises a transition from pelagic to benthic form, which involves considerable physiological and structural changes and has vital roles in its natural populations and commercial breeding. Thus, understanding the endogenous microRNAs (miRNAs) that drive metamorphosis is of great interest. This is the first study to use high-throughput sequencing to examine the alterations in miRNA expression that occur during metamorphosis in a marine gastropod. A total of 195 differentially expressed miRNAs were obtained. Sixty-five of these were expressed during the transition from precompetent to competent larvae. Thirty-three of these were upregulated and the others were downregulated. Another 123 miRNAs were expressed during the transition from competent to postlarvae. Ninety-six of these were upregulated and the remaining 27 were downregulated. The expression ofmiR-276-y, miR-100-x, miR-183-x, and miR-263-x showed a >100-fold change during development, while the miR-242-x and novel-m0052-3p expression levels changed over 3000-fold. Putative target gene coexpression, gene ontology, and pathway analyses suggest that these miRNAs play important parts in cell proliferation, migration, apoptosis, metabolic regulation, and energy absorption. Twenty miRNAs and their target genes involved in ingestion, digestion, cytoskeleton, cell adhesion, and apoptosis were identified. Nine of them were analyzed with real-time polymerase chain reaction (PCR), which showed an inverse correlation between the miRNAs and their relative expression levels. Our data elucidate the role of miRNAs in R. venosa metamorphic transition and serve as a solid basis for further investigations into regulatory mechanisms of gastropod metamorphosis.
关键词	miRNA metamorphic transition gastropod larval
DOI	10.1534/g3.117.300210
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China (NSFC)[31572636] ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; Primary Research & Development Plan of Shandong Province[2016ZDJS06A02] ; Industry Leading Talent Project of Taishan scholars ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020703] ; National Natural Science Foundation of China (NSFC)[31572636] ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; Primary Research & Development Plan of Shandong Province[2016ZDJS06A02] ; Industry Leading Talent Project of Taishan scholars ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020703] ; National Natural Science Foundation of China (NSFC)[31572636] ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; Primary Research & Development Plan of Shandong Province[2016ZDJS06A02] ; Industry Leading Talent Project of Taishan scholars ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020703]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000417073900016
出版者	GENETICS SOCIETY AMERICA
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157374
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Tao; Wang, Hai-yan
作者单位	1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ocean Univ China, Coll Fisheries, Qingdao 266001, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Song, Hao,Qi, Lu,Zhang, Tao,et al. Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)[J]. G3-GENES GENOMES GENETICS,2017,7(12):3999-4008.
APA	Song, Hao,Qi, Lu,Zhang, Tao,&Wang, Hai-yan.(2017).Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa).G3-GENES GENOMES GENETICS,7(12),3999-4008.
MLA	Song, Hao,et al."Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)".G3-GENES GENOMES GENETICS 7.12(2017):3999-4008.