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ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection
Liu, Peng-fei1,2; Du, Yishuai1; Meng, Lingjie1,2; Li, Xian1; Liu, Ying1,3
2017-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume70Pages:30-39
Corresponding AuthorLiu, Ying(yingliu@dlou.edu.cn)
AbstractAldehyde dehydrogenases (ALDHs) belong to a super-family of detoxifying proteins and perform a significant role in developing epithelial homeostasis, protecting cells from toxic aldehydes and drug resistance. However, the activity and function of these detoxifying proteins remain unknown, especially in fish. In our research, we aimed to study functions of aldehyde dehydrogenase 7A1 (ALDH7A1) in Atlantic salmon infected by Aeromonas salmonicida. Recombinant ALDH7A1 (rALDH7A1) was verified by SDS-PAGE and western blot. The molecular mass of the deduced amino acid sequence of rALDH7A1 is 58.9 kDa with an estimated pI of 7.09. Only a low complexity region ((141)yvegvgevqeyvdv(153)) without a signal peptide existed in rALDH7A1. Results of ELISA indicated that rALDH7A1 exhibited apparent binding activities with A. salmonicida and its expression was highest in fish kidney. A Real Time PCR (qRT-PCR) assay in kidneys confirmed that fish in this experiment were authentically infected and bacterial loads in rALDH7A1-adminsitered fish were significantly reduced at an early stage of infection. Meanwhile, we found the mRNA expression of NF-k beta, P-38 MAPK, caspase-3 and TNF-alpha were mainly up regulated at 72 h in the kidneys and livers of highly infected fish injected with rALDH7A1, and the same variation trend existed in fish spleens at 12 h. Consistent with these observations, neutralization experiments in vivo indicated that rALDH7A1 could obviously reduce the death rate compared to the BSA and control group. Taken together, we concluded that rALDH7A1 could act in host immune defense against bacterial infection and decrease the mortality rate of Atlantic salmon at early stages of infection with A. salmonicida. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordALDH7A1 Aeromonas salmonicida Atlantic salmon Recombinant protein
DOI10.1016/j.fsi.2017.08.043
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Fund of China[41306152] ; National Natural Science Fund of China[31472312] ; National Natural Science Fund of China[31402283] ; key R&D Program of Jiangsu Province[BE2015325] ; earmarked fund for the Modern Agro-industry Technology Research System[CARS 48]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000416186800004
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/157315
Collection中国科学院海洋研究所
Corresponding AuthorLiu, Ying
Affiliation1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Dalian Ocean Univ, Dalian, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,et al. ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection[J]. FISH & SHELLFISH IMMUNOLOGY,2017,70:30-39.
APA Liu, Peng-fei,Du, Yishuai,Meng, Lingjie,Li, Xian,&Liu, Ying.(2017).ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection.FISH & SHELLFISH IMMUNOLOGY,70,30-39.
MLA Liu, Peng-fei,et al."ALDH7A1 is a protein that protects Atlantic salmon against Aeromonas salmonicida at the early stages of infection".FISH & SHELLFISH IMMUNOLOGY 70(2017):30-39.
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