Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum | |
Jiang, Shuai1; Wang, Lingling2; Huang, Mengmeng1 ; Jia, Zhihao1; Weinert, Tobias3; Warkentin, Eberhard4; Liu, Conghui1; Song, Xiaorui1; Zhang, Haixia1; Witt, Jennifer4; Qiu, Limei1; Peng, Guohong1,4; Song, Linsheng2
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| 2017-11-29 | |
| Source Publication | FRONTIERS IN IMMUNOLOGY
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| ISSN | 1664-3224 |
| Volume | 8Pages:17 |
| Corresponding Author | Peng, Guohong(guohong.peng@biophys.mpg.de) ; Song, Linsheng(lshsong@dlou.edu.cn) |
| Abstract | DM9 domain was first identified in Drosophila melanogaster, and it was subsequently found to integrate with or without other protein domains across a wide range of invertebrates and vertebrates. In the present study, a member of DM9 domain containing protein (DM9CP) family from marine invertebrate Crassostrea gigas (designated CgDM9CP-1), which was only composed of two DM9 domains, was taken as a protein model to study the biological functions of DM9 domain and its molecular determinants. CgDM9CP-1 was found to exhibit high binding specificity and avidity toward D-mannose residue. It served as a pattern recognition receptor (PRR) with a broad range of recognition spectrum to various pathogen-associated molecular patterns, including lipopolysaccharide, peptidylglycan, mannan, and beta-1, 3-glucan in a D-mannose-dependent manner, as well as bacteria and fungi. In order to reveal the molecular mechanism underlying its pattern recognition activity, the crystal structures of wild-type and loss-of-function mutants were solved, and Asp22 and Lys43 were found to be the critical residues for ligand recognition. Moreover, CgDM9CP-1 protein was found to mainly distribute on the surface of C. gigas hemocytes, and it could be translocated into cytoplasm and colocalized with the engulfed microbes during hemocyte phagocytosis. The present result clearly indicated that CgDM9CP-1 was a PRR, and it provided an important clue for the better understanding of DM9CP function. |
| Keyword | innate immunity pattern recognition receptor DM9 domain crystal structure mannose binding phagocytosis |
| DOI | 10.3389/fimmu.2017.01607 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Natural Science Foundation of China[31530069] ; Natural Science Foundation of China[41406170] ; High Technology Project (863 Program) from the Chinese Ministry of Science and Technology[2014AA093501] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; Max-Planck-Gesellschaft ; Natural Science Foundation of China[31530069] ; Natural Science Foundation of China[41406170] ; High Technology Project (863 Program) from the Chinese Ministry of Science and Technology[2014AA093501] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; Max-Planck-Gesellschaft ; Natural Science Foundation of China[31530069] ; Natural Science Foundation of China[41406170] ; High Technology Project (863 Program) from the Chinese Ministry of Science and Technology[2014AA093501] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] ; Max-Planck-Gesellschaft |
| WOS Research Area | Immunology |
| WOS Subject | Immunology |
| WOS ID | WOS:000416404600001 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/157306 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Peng, Guohong; Song, Linsheng |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol Dis Control, Dalian, Peoples R China 3.Paul Scherrer Inst, Lab Biomol Res, Villigen, Switzerland 4.Max Planck Inst Biophys, Dept Mol Membrane Biol, Frankfurt, Germany |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Jiang, Shuai,Wang, Lingling,Huang, Mengmeng,et al. DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum[J]. FRONTIERS IN IMMUNOLOGY,2017,8:17. |
| APA | Jiang, Shuai.,Wang, Lingling.,Huang, Mengmeng.,Jia, Zhihao.,Weinert, Tobias.,...&Song, Linsheng.(2017).DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum.FRONTIERS IN IMMUNOLOGY,8,17. |
| MLA | Jiang, Shuai,et al."DM9 Domain Containing Protein Functions As a Pattern Recognition Receptor with Broad Microbial Recognition Spectrum".FRONTIERS IN IMMUNOLOGY 8(2017):17. |
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