Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Energy response and modulation of AMPK pathway of the olive flounder Paralichthys olivaceus in low-temperature challenged | |
Lu, Yunliang1,2; Nie, Miaomiao1,2; Wang, Ling1,2; Xiong, Yinghuai3,4; Wang, Fang3,4; Wang, Lijuan1,2 ; Xiao, Peng1,2; Wu, Zhihao1,2; Liu, Ying5; You, Feng1,2
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| 2018-02-01 | |
| Source Publication | AQUACULTURE
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| ISSN | 0044-8486 |
| Volume | 484Pages:205-213 |
| Corresponding Author | You, Feng(youfeng@qdio.ac.cn) |
| Abstract | Cold resistance is important for fish in natural and aquaculture environments. Modulation of energy metabolism has been proved to be vital for fish in cold. However, the interaction of energy response and the known AMPK pathway remains vague in fish coping with cold stress. In the present study, the olive flounders Paralichthys olivaceus were subject to low-temperature condition (0.2 +/- 0.2 degrees C). The energy metabolism and modulation of AMPK pathway were analyzed. Results showed that flounders increased ATP-coupled ion transport, as indicated by Na+/K+-ATPase and Ca2+/Mg2+-ATPase activities. Nevertheless, higher levels of aerobic and anaerobic metabolism, respectively indicated by the elevation of COX and LDH activities, contributed to the stable energy homeostasis during cold exposure. The three subunits of AMPK responded differently to cold, and AMPK alpha and AMPK beta might be more sensitive in the early stage while AMPK gamma might be more sensitive in the later stage. The transcriptional response of LKB1/CaMKK beta-AMPK-NRF1 axis might play an important role in helping flounders against the early cold stress. However, flounders differentiated in cold resistance with the stress time elapsed. Concomitantly a greater reliance on LKB1-AMPK-NRF1 axis for cold-tolerant flounders compared to those sensitive ones to cold was observed, and this axis was speculated to be related with flounder cold resistance. To our knowledge, this is the first time to explore the possible response of AMPK pathway to cold in fish. Our data showed that the flounder relied on metabolic regulation and modulation of AMPK pathway including not only higher expression levels of genes in this pathway but also changes in its related function axes to maintain energy homeostasis. |
| Keyword | Paralichthys olivaceus Cold stress Energy response Metabolic regulation AMPK pathway |
| DOI | 10.1016/j.aquaculture.2017.11.031 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31602143] ; China Postdoctoral Science Foundation[2016M602202] ; National 863 High Technology Research Foundation of China[2012AA10A408] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Flatfish Industry System Construction Programme of China[nycytx-50] ; National Infrastructure of Fishery Germplasm Resources ; National Natural Science Foundation of China[31602143] ; China Postdoctoral Science Foundation[2016M602202] ; National 863 High Technology Research Foundation of China[2012AA10A408] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Flatfish Industry System Construction Programme of China[nycytx-50] ; National Infrastructure of Fishery Germplasm Resources ; National Natural Science Foundation of China[31602143] ; China Postdoctoral Science Foundation[2016M602202] ; National 863 High Technology Research Foundation of China[2012AA10A408] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Flatfish Industry System Construction Programme of China[nycytx-50] ; National Infrastructure of Fishery Germplasm Resources |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000416741800027 |
| Publisher | ELSEVIER SCIENCE BV |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/157297 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | You, Feng |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Shandong, Peoples R China 3.Ocean Univ China, Minist Educ, Key Lab Mariculture, Qingdao 266003, Shandong, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266200, Shandong, Peoples R China 5.Dalian Ocean Univ, Sch Marine Sci & Environm Engn, Dalian 116023, Liaoning, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Lu, Yunliang,Nie, Miaomiao,Wang, Ling,et al. Energy response and modulation of AMPK pathway of the olive flounder Paralichthys olivaceus in low-temperature challenged[J]. AQUACULTURE,2018,484:205-213. |
| APA | Lu, Yunliang.,Nie, Miaomiao.,Wang, Ling.,Xiong, Yinghuai.,Wang, Fang.,...&You, Feng.(2018).Energy response and modulation of AMPK pathway of the olive flounder Paralichthys olivaceus in low-temperature challenged.AQUACULTURE,484,205-213. |
| MLA | Lu, Yunliang,et al."Energy response and modulation of AMPK pathway of the olive flounder Paralichthys olivaceus in low-temperature challenged".AQUACULTURE 484(2018):205-213. |
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| Energy response and （1221KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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