IOCAS-IR  > 实验海洋生物学重点实验室
A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1
Wang, M.1; Wang, B.1; Liu, M.1; Jiang, K.1; Wang, L.1,2
2017
发表期刊ISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN1824-307X
卷号14页码:453-463
通讯作者Wang, L.(wanglei@qdio.ac.cn)
摘要Macrophage migration inhibitory factor (MIF) was an ancient cytokine and involved in innate immunity of vertebrates and invertebrates. In the present study, a novel MIF homologue (designated as LvMIF2) has been cloned from the Pacific white shrimp Litopenaeus vannamei via rapid amplification of cDNA ends (RACE) technique. The full-length cDNA sequence of LvMIF2 was 555 bp and contained a 97 bp 5' untranslated region (UTR) and a 3' UTR of 110 bp, and an open reading frame (ORF) of 348 bp which coded 115 amino acids. Quantitative realtime PCR (qRT-PCR) analysis indicated that LvMIF2 was constitutively expressed in all the tested tissues, including eyestalk, gill, gonad, heart, hemocytes, hepatopancreas, intestine, muscle, nerve and stomach, with the highest mRNA expression level in hemocytes and hepatopancreas. After Vibrio parahaemolyticus or white spot syndrome virus (WSSV) challenge, the mRNA expression levels of LvMIF2 in hemocytes and hepatopancreas were all sharply upregulated. Comparative sequence and transcription analysis between the previously identified LvMIF1 and LvMIF2 revealed that both LvMIF1 and LvMIF2 might play crucial and functionally differentiated roles in shrimp innate immune responses to bacterial and viral stimulation.
关键词Litopenaeus vannamei macrophage migration inhibitory factor innate immunity
收录类别SCI
语种英语
资助项目Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002]
WOS研究方向Immunology ; Zoology
WOS类目Immunology ; Zoology
WOS记录号WOS:000416558800003
出版者INVERTEBRATE SURVIVAL JOURNAL
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/157276
专题实验海洋生物学重点实验室
通讯作者Wang, L.
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
第一作者单位实验海洋生物学重点实验室
通讯作者单位实验海洋生物学重点实验室
推荐引用方式
GB/T 7714
Wang, M.,Wang, B.,Liu, M.,et al. A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2017,14:453-463.
APA Wang, M.,Wang, B.,Liu, M.,Jiang, K.,&Wang, L..(2017).A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1.ISJ-INVERTEBRATE SURVIVAL JOURNAL,14,453-463.
MLA Wang, M.,et al."A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1".ISJ-INVERTEBRATE SURVIVAL JOURNAL 14(2017):453-463.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, M.]的文章
[Wang, B.]的文章
[Liu, M.]的文章
百度学术
百度学术中相似的文章
[Wang, M.]的文章
[Wang, B.]的文章
[Liu, M.]的文章
必应学术
必应学术中相似的文章
[Wang, M.]的文章
[Wang, B.]的文章
[Liu, M.]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。