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A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1
Wang, M.1; Wang, B.1; Liu, M.1; Jiang, K.1; Wang, L.1,2
2017
Source PublicationISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN1824-307X
Volume14Pages:453-463
Corresponding AuthorWang, L.(wanglei@qdio.ac.cn)
AbstractMacrophage migration inhibitory factor (MIF) was an ancient cytokine and involved in innate immunity of vertebrates and invertebrates. In the present study, a novel MIF homologue (designated as LvMIF2) has been cloned from the Pacific white shrimp Litopenaeus vannamei via rapid amplification of cDNA ends (RACE) technique. The full-length cDNA sequence of LvMIF2 was 555 bp and contained a 97 bp 5' untranslated region (UTR) and a 3' UTR of 110 bp, and an open reading frame (ORF) of 348 bp which coded 115 amino acids. Quantitative realtime PCR (qRT-PCR) analysis indicated that LvMIF2 was constitutively expressed in all the tested tissues, including eyestalk, gill, gonad, heart, hemocytes, hepatopancreas, intestine, muscle, nerve and stomach, with the highest mRNA expression level in hemocytes and hepatopancreas. After Vibrio parahaemolyticus or white spot syndrome virus (WSSV) challenge, the mRNA expression levels of LvMIF2 in hemocytes and hepatopancreas were all sharply upregulated. Comparative sequence and transcription analysis between the previously identified LvMIF1 and LvMIF2 revealed that both LvMIF1 and LvMIF2 might play crucial and functionally differentiated roles in shrimp innate immune responses to bacterial and viral stimulation.
KeywordLitopenaeus vannamei macrophage migration inhibitory factor innate immunity
Indexed BySCI
Language英语
Funding ProjectKey Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Major Projects of Shandong Province[2015ZDZX05002]
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000416558800003
PublisherINVERTEBRATE SURVIVAL JOURNAL
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/157276
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, L.
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, M.,Wang, B.,Liu, M.,et al. A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2017,14:453-463.
APA Wang, M.,Wang, B.,Liu, M.,Jiang, K.,&Wang, L..(2017).A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1.ISJ-INVERTEBRATE SURVIVAL JOURNAL,14,453-463.
MLA Wang, M.,et al."A novel macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (LvMIF2): comparative sequence and transcription analysis with LvMIF1".ISJ-INVERTEBRATE SURVIVAL JOURNAL 14(2017):453-463.
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