Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China

doi:10.1016/j.hal.2019.03.011

	Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China
	Chen, Zhen-Fan 1,3; Zhang, Qing-Chun 1,2,4; Kong, Fan-Zhou1,2,4 ; Liu, Yang5 ; Zhao, Yue 1,3; Zhou, Zheng-Xi1 ; Geng, Hui-Xia1 ; Dai, Li 1,3; Zhou, Ming-Jiang 1; Yu, Ren-Cheng 1,2,3,4
	2019-04-01
发表期刊	HARMFUL ALGAE
ISSN	1568-9883
卷号	84 页码:127-138
通讯作者	Zhang, Qing-Chun(qczhang@qdio.ac.cn) ; Yu, Ren-Cheng(rcyu@qdio.ac.cn)
摘要	Large-scale blooms formed by pico-sized phytoplankton, which strongly inhibited feeding activity and growth of cultured scallops, have been recorded along the coast of Qinhuangdao in the Bohai Sea since 2009. Based on pigment profiles and clone library analysis of phytoplankton samples during the blooms, the major bloom-forming species was primarily identified as pelagophyte Aureococcus anophagefferens Hargraves et Sieburth, the causative species of intensive brown tides in the United States and South Africa. Due to the indistinct morphological features of the bloom-forming microalgae and limited knowledge on the composition of phytoplankton communities, there were still disputes concerning the causative species of the blooms. In this study, the method of high-throughput sequencing targeted 18S rDNA V4 region was used to study the composition of pico- and nano-sized phytoplankton communities in 2013 and 2014. A total of 18 groups of eukaryotic microalgae at the class level and more than 2000 operational taxonomic units (OTUs) were identified in phytoplankton samples collected from the brown-tide zone in the Qinhuangdao coastal waters. For both years, A. anophagefferens was the most dominant species during the bloom period and its maximum relative abundance exceeded 60 percent. Along with other evidence, the results further confirm that A. anophagefferens is the major causative species of the pico-sized phytoplankton blooms in the Bohai Sea. The outbreak of brown tide exhibited a strong inter-annual variation between 2013 and 2014, and an increasing dominance of dinoflagellates could be observed in the Qinhuangdao coastal waters.
关键词	Brown tide Aureococcus anophagefferens High-throughput sequencing Phytoplankton community
DOI	10.1016/j.hal.2019.03.011
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41576121] ; National Natural Science Foundation of China[41276117] ; National Natural Science Foundation of China[U1606404] ; Program of the Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02] ; National Natural Science Foundation of China[41576121] ; National Natural Science Foundation of China[41276117] ; National Natural Science Foundation of China[U1606404] ; Program of the Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02]
WOS研究方向	Marine & Freshwater Biology
WOS类目	Marine & Freshwater Biology
WOS记录号	WOS:000471087800014
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/156856
专题	海洋生态与环境科学重点实验室
通讯作者	Zhang, Qing-Chun; Yu, Ren-Cheng
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 5.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chen, Zhen-Fan,Zhang, Qing-Chun,Kong, Fan-Zhou,et al. Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China[J]. HARMFUL ALGAE,2019,84:127-138.
APA	Chen, Zhen-Fan.,Zhang, Qing-Chun.,Kong, Fan-Zhou.,Liu, Yang.,Zhao, Yue.,...&Yu, Ren-Cheng.(2019).Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China.HARMFUL ALGAE,84,127-138.
MLA	Chen, Zhen-Fan,et al."Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China".HARMFUL ALGAE 84(2019):127-138.

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