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Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China
Chen, Zhen-Fan1,3; Zhang, Qing-Chun1,2,4; Kong, Fan-Zhou1,2,4; Liu, Yang5; Zhao, Yue1,3; Zhou, Zheng-Xi1; Geng, Hui-Xia1; Dai, Li1,3; Zhou, Ming-Jiang1; Yu, Ren-Cheng1,2,3,4
2019-04-01
Source PublicationHARMFUL ALGAE
ISSN1568-9883
Volume84Pages:127-138
Corresponding AuthorZhang, Qing-Chun(qczhang@qdio.ac.cn) ; Yu, Ren-Cheng(rcyu@qdio.ac.cn)
AbstractLarge-scale blooms formed by pico-sized phytoplankton, which strongly inhibited feeding activity and growth of cultured scallops, have been recorded along the coast of Qinhuangdao in the Bohai Sea since 2009. Based on pigment profiles and clone library analysis of phytoplankton samples during the blooms, the major bloom-forming species was primarily identified as pelagophyte Aureococcus anophagefferens Hargraves et Sieburth, the causative species of intensive brown tides in the United States and South Africa. Due to the indistinct morphological features of the bloom-forming microalgae and limited knowledge on the composition of phytoplankton communities, there were still disputes concerning the causative species of the blooms. In this study, the method of high-throughput sequencing targeted 18S rDNA V4 region was used to study the composition of pico- and nano-sized phytoplankton communities in 2013 and 2014. A total of 18 groups of eukaryotic microalgae at the class level and more than 2000 operational taxonomic units (OTUs) were identified in phytoplankton samples collected from the brown-tide zone in the Qinhuangdao coastal waters. For both years, A. anophagefferens was the most dominant species during the bloom period and its maximum relative abundance exceeded 60 percent. Along with other evidence, the results further confirm that A. anophagefferens is the major causative species of the pico-sized phytoplankton blooms in the Bohai Sea. The outbreak of brown tide exhibited a strong inter-annual variation between 2013 and 2014, and an increasing dominance of dinoflagellates could be observed in the Qinhuangdao coastal waters.
KeywordBrown tide Aureococcus anophagefferens High-throughput sequencing Phytoplankton community
DOI10.1016/j.hal.2019.03.011
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41576121] ; National Natural Science Foundation of China[41276117] ; National Natural Science Foundation of China[U1606404] ; Program of the Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02]
WOS Research AreaMarine & Freshwater Biology
WOS SubjectMarine & Freshwater Biology
WOS IDWOS:000471087800014
PublisherELSEVIER SCIENCE BV
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156856
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhang, Qing-Chun; Yu, Ren-Cheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
5.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Zhen-Fan,Zhang, Qing-Chun,Kong, Fan-Zhou,et al. Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China[J]. HARMFUL ALGAE,2019,84:127-138.
APA Chen, Zhen-Fan.,Zhang, Qing-Chun.,Kong, Fan-Zhou.,Liu, Yang.,Zhao, Yue.,...&Yu, Ren-Cheng.(2019).Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China.HARMFUL ALGAE,84,127-138.
MLA Chen, Zhen-Fan,et al."Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China".HARMFUL ALGAE 84(2019):127-138.
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