IOCAS-IR  > 海洋生态与环境科学重点实验室
Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China
Zhen-Fan Chen1,3; Qing-Chun Zhang1,2,4; Fan-Zhou Kong1,2,4; Yang Liu5; Yue Zhao1,3; Zheng-Xi Zhou1; Hui-Xia Geng1; Li Dai1,3; Ming-Jiang Zhou1; Ren-Cheng Yu1,2,3,4
2019-04
Source PublicationHarmful Algae
Volume84Issue:4Pages:127-138
Abstract

Large-scale blooms formed by pico-sized phytoplankton, which strongly inhibited feeding activity and growth of cultured scallops, have been recorded along the coast of Qinhuangdao in the Bohai Sea since 2009. Based on pigment profiles and clone library analysis of phytoplankton samples during the blooms, the major bloom-forming species was primarily identified as pelagophyte Aureococcus anophagefferens Hargraves et Sieburth, the causative species of intensive brown tides in the United States and South Africa. Due to the indistinct morphological features of the bloom-forming microalgae and limited knowledge on the composition of phytoplankton communities, there were still disputes concerning the causative species of the blooms. In this study, the method of high-throughput sequencing targeted 18S rDNA V4 region was used to study the composition of pico- and nano-sized phytoplankton communities in 2013 and 2014. A total of 18 groups of eukaryotic microalgae at the class level and more than 2,000 operational taxonomic units (OTUs) were identified in phytoplankton samples collected from the brown-tide zone in the Qinhuangdao coastal waters. For both years, A. anophagefferens was the most dominant species during the bloom period and its maximum relative abundance exceeded 60 percent. Along with other evidence, the results further confirm that A. anophagefferens is the major causative species of the pico-sized phytoplankton blooms in the Bohai Sea. The outbreak of brown tide exhibited a strong inter-annual variation between 2013 and 2014, and an increasing dominance of dinoflagellates could be observed in the Qinhuangdao coastal waters.

Keywords: Brown tide, Aureococcus anophagefferens, High-throughput sequencing, Phytoplankton community

KeywordBrown Tide Aureococcus Anophagefferens High-throughput Sequencing Phytoplankton Community
MOST Discipline Catalogue理学 ; 理学::海洋科学
Indexed BySCI
Document Type期刊论文
Version出版稿
Identifierhttp://ir.qdio.ac.cn/handle/337002/156856
Collection海洋生态与环境科学重点实验室
Corresponding AuthorQing-Chun Zhang; Ren-Cheng Yu
Affiliation1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China
2.Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China
3.University of Chinese Academy of Sciences, Beijing, 100049, China
4.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China
5.CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences, Guangzhou, 510301, China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhen-Fan Chen,Qing-Chun Zhang,Fan-Zhou Kong,et al. Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China[J]. Harmful Algae,2019,84(4):127-138.
APA Zhen-Fan Chen.,Qing-Chun Zhang.,Fan-Zhou Kong.,Yang Liu.,Yue Zhao.,...&Ren-Cheng Yu.(2019).Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China.Harmful Algae,84(4),127-138.
MLA Zhen-Fan Chen,et al."Resolving phytoplankton taxa based on high-throughput sequencing during brown tides in the Bohai Sea, China".Harmful Algae 84.4(2019):127-138.
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