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Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation
Wang, Xueying1,2; Liu, Qinghua1,2; Xu, Shihong1,2; Xiao, Yongshuang1,2; Wang, Yanfeng1,2; Feng, Chengcheng1,2; Xue, Rui1,2,3; Zhao, Haixia1,2,3; Song, Zongcheng4; Li, Jun1,2
2018-10-25
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume8Pages:12
Corresponding AuthorLiu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
AbstractSpermatogenesis is a dynamic developmental process in which spermatogonial stem cells proliferate, differentiate and mature into functional spermatozoa. These processes require an accurate gene regulation network. Here, we investigated the dynamic changes that occur during spermatogenesis through a combination of histological and transcriptome analyses of different developmental stages of the testis. We constructed 18 testis transcriptome libraries, and the average length, N50, and GC content of the unigenes were 1,795 bp; 3,240 bp and 49.25%, respectively. Differentially expressed genes (DEGs) that were related to germ cell proliferation and maturation, such as NANOS3, RARs, KIFs, steroid hormone synthesis-related genes and receptor genes, were identified between pairs of testis at different developmental stages. Gene ontology annotation and pathway analyses were conducted on DEGs with specific expression patterns involved in the regulation of spermatogenesis. Nine important pathways such as steroid hormone biosynthesis related to spermatogenesis were identified. A total of 21 modules that ranged from 49 to 7,448 genes were designed by a weighted gene co-expression network analysis. Furthermore, a total of 83 candidate miRNA were identified by computational methods. Our study provides the first transcriptomic evidence for differences in gene expression between different developmental stages of spermatogenesis in turbot (Scophthalmus maximus).
DOI10.1038/s41598-018-34149-5
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31472264] ; National Natural Science Foundation of China[31572602] ; National Natural Science Foundation of China[31372514] ; China Agriculture Research System[CARS-47] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03-03] ; Chinese Academy of science and technology service network planning[KFJ-EW-STS-060] ; Youth Innovation Promotion Association CAS ; Scientific and Technological Innovation Project
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000448271600024
PublisherNATURE PUBLISHING GROUP
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156569
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Qinghua; Li, Jun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Xueying,Liu, Qinghua,Xu, Shihong,et al. Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation[J]. SCIENTIFIC REPORTS,2018,8:12.
APA Wang, Xueying.,Liu, Qinghua.,Xu, Shihong.,Xiao, Yongshuang.,Wang, Yanfeng.,...&Li, Jun.(2018).Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation.SCIENTIFIC REPORTS,8,12.
MLA Wang, Xueying,et al."Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation".SCIENTIFIC REPORTS 8(2018):12.
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