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Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites
Li, Meng1,2; Huang, Qian1,2,3; Wang, Jinfeng1,2,3; Li, Caiwen1,2,4
2018-12-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume83Pages:134-139
Corresponding AuthorLi, Caiwen(caiwenli@live.com)
AbstractLatest studies have indicated that microRNAs (miRNAs) play important roles in defending against bacterial and viral infections in marine crustacean, whereas little is known regarding the immunological roles of crustacean miRNAs in response to parasitic infection. To further reveal the host-parasite interactions between the parasitic dinoflagellate Hematodinium and its crustacean hosts, we applied the high-throughput sequencing technology to identify and characterize miRNAs in the Chinese swimming crab Portunus trituberculatus challenged with the Hematodinium parasite at a timescale of 16 days (d). A total of 168 miRNAs were identified and 51 miRNAs were differentially expressed in the hepatopancreas tissues of affected hosts. Eleven of the differentially expressed miRNAs were selected and verified by the quantitative real-time RT-PCR (qRT-PCR), manifesting the consistency between the high throughout sequencing and qRT-PCR assays. Further analysis of the putative target genes indicated that various immune-related pathways (e.g. endocytosis, Fc gamma R-mediated phagocytosis, lysosome, ECM-receptor interaction, complement and coagulation cascades, antigen processing and presentation, focal adhesion, etc.) and signal transduction pathways (e.g. JAK-STAT signaling pathway, MAPK signaling pathway, p53 signaling pathway, etc.) were mediated by the differentially expressed miRNAs. The results presented fundamental knowledge on the immunological roles of crustacean miRNAs and contributed to the better understanding of hosts' miRNAs-mediated immunity against the parasitic infection.
KeywordMarine crustacean Parasite Innate immunity microRNA Host-parasite interaction
DOI10.1016/j.fsi.2018.09.011
Indexed BySCI
Language英语
Funding ProjectGeneral Program of National Nature Science Foundation of China (NSFC)[41676102] ; CPSF-CAS Joint Foundation for Excellent Postdoctoral Fellows[2016LH0034] ; China Postdoctoral Science Foundation[2016M600561]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000448096200015
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156533
Collection海洋生态与环境科学重点实验室
Corresponding AuthorLi, Caiwen
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Corresponding Author AffilicationKey Laboratory of Marine Ecology & Environmental Sciences, CAS
Recommended Citation
GB/T 7714
Li, Meng,Huang, Qian,Wang, Jinfeng,et al. Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites[J]. FISH & SHELLFISH IMMUNOLOGY,2018,83:134-139.
APA Li, Meng,Huang, Qian,Wang, Jinfeng,&Li, Caiwen.(2018).Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites.FISH & SHELLFISH IMMUNOLOGY,83,134-139.
MLA Li, Meng,et al."Differential expression of microRNAs in Portunus trituberculatus in response to Hematodinium parasites".FISH & SHELLFISH IMMUNOLOGY 83(2018):134-139.
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