IOCAS-IR  > 海洋生物分类与系统演化实验室
DNA barcoding of the family Sparidae along the coast of China and revelation of potential cryptic diversity in the Indo-West Pacific oceans based on COI and 16S rRNA genes
Wu Renxie1; Zhang Haoran1; Liu Jing2; Niu Sufang1; Xiao Yao1; Chen Yongxia3
2018-09-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:5Pages:1753-1770
Corresponding AuthorLiu Jing(jliu@qdio.ac.cn)
AbstractSparids are of considerable economic importance in marine fishery and aquaculture in China, and the species diversity of this group is considered relatively high. However, the accurate species identification and delimitation of sparids in China remain unaddressed. In this study, we used mitochondrial cytochrome oxidase subunit. (COI) and 16S ribosomal RNA (16S) genes to conduct DNA barcoding and species delimitation in eleven sparid species from the coastal waters of China. Based on Kimura-2 parameter genetic distances, the mean intraspecific/interspecific variation for COI and 16S were calculated as 0.004/0.152 and 0.002/0.072, respectively. All the conspecific individuals formed monophyletic clusters in neighbour-joining trees of both markers. An obvious barcoding gap was detected for each species, and a common genetic threshold of 1.3% sequence divergence was defined for species delimitation in both markers. Although the sequence variation of 16S was generally lower than that of COI, the results indicated that sparid species could be effectively and accurately identified and delimited by COI as well as 16S. Thus, we propose that the COI gene serve as the standard DNA barcode for sparids, and that the 16S gene could also be an ideal candidate barcode. Moreover, each of the six sparid species (Argyrops spinifer, Rhabdosargus sarba, Dentex hypselosomus, Acanthopagrus latus, Acanthopagrus australis and Acanthopagrus berda) showed high intraspecifi c divergence (>1.3% genetic threshold) with the remarkable geographic lineages in the Indo-West Pacific oceans, which supported that potential unrecognized cryptic species were in them. The potential cryptic diversity revealed here might be primarily attributed to the allopatric divergences caused by the long-term geographic isolation between the Indian and West Pacific oceans or between the opposite sides of the Indian Ocean. The results further suggest that a revision of taxonomic status of these species is required, followed by development of a biodiversity conservation strategy.
KeywordSparidae DNA barcoding species delimitation cryptic species Chinese coast Indo-West Pacific
DOI10.1007/s00343-018-7214-6
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31372532] ; National Natural Science Foundation of China[41006084] ; National Natural Science Foundation of China[41276166] ; Project for Outstanding Young Teachers in Higher Education of Guangdong, China[Yq2013093]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000450165300026
PublisherSCIENCE PRESS
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156419
Collection海洋生物分类与系统演化实验室
Corresponding AuthorLiu Jing
Affiliation1.Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China
3.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu Renxie,Zhang Haoran,Liu Jing,et al. DNA barcoding of the family Sparidae along the coast of China and revelation of potential cryptic diversity in the Indo-West Pacific oceans based on COI and 16S rRNA genes[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(5):1753-1770.
APA Wu Renxie,Zhang Haoran,Liu Jing,Niu Sufang,Xiao Yao,&Chen Yongxia.(2018).DNA barcoding of the family Sparidae along the coast of China and revelation of potential cryptic diversity in the Indo-West Pacific oceans based on COI and 16S rRNA genes.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(5),1753-1770.
MLA Wu Renxie,et al."DNA barcoding of the family Sparidae along the coast of China and revelation of potential cryptic diversity in the Indo-West Pacific oceans based on COI and 16S rRNA genes".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.5(2018):1753-1770.
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