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High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)
Wu Chunhui1,2,3; Jiang Peng1,2; Zhao Jin1,2; Fu Huihui1,2
2018-09-01
发表期刊JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
卷号36期号:5页码:1806-1811
通讯作者Jiang Peng(jiangpeng@qdio.ac.cn)
摘要Protoplast isolation was relevant for gene manipulation in Ulva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transfomiation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. NeitherF(v)/F-m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an effective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast ((31.5 +/- 1.9)x 10(6) cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.
关键词protoplast Uhva prolifera regeneration artificial culture
DOI10.1007/s00343-018-7058-0
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[41776153] ; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02-1] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020304] ; Post-doctoral Application Research Program - Qingdao[2016189] ; National Natural Science Foundation of China[41776153] ; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02-1] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020304] ; Post-doctoral Application Research Program - Qingdao[2016189]
WOS研究方向Marine & Freshwater Biology ; Oceanography
WOS类目Limnology ; Oceanography
WOS记录号WOS:000450165300031
出版者SCIENCE PRESS
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/156409
专题实验海洋生物学重点实验室
通讯作者Jiang Peng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位实验海洋生物学重点实验室
通讯作者单位实验海洋生物学重点实验室
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Wu Chunhui,Jiang Peng,Zhao Jin,et al. High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(5):1806-1811.
APA Wu Chunhui,Jiang Peng,Zhao Jin,&Fu Huihui.(2018).High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta).JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(5),1806-1811.
MLA Wu Chunhui,et al."High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.5(2018):1806-1811.
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