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High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)
Wu Chunhui1,2,3; Jiang Peng1,2; Zhao Jin1,2; Fu Huihui1,2
2018-09-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:5Pages:1806-1811
Corresponding AuthorJiang Peng(jiangpeng@qdio.ac.cn)
AbstractProtoplast isolation was relevant for gene manipulation in Ulva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transfomiation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. NeitherF(v)/F-m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an effective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast ((31.5 +/- 1.9)x 10(6) cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.
Keywordprotoplast Uhva prolifera regeneration artificial culture
DOI10.1007/s00343-018-7058-0
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41776153] ; Qingdao National Laboratory for Marine Science and Technology[2016ASKJ02-1] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020304] ; Post-doctoral Application Research Program - Qingdao[2016189]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000450165300031
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156409
Collection实验海洋生物学重点实验室
Corresponding AuthorJiang Peng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu Chunhui,Jiang Peng,Zhao Jin,et al. High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(5):1806-1811.
APA Wu Chunhui,Jiang Peng,Zhao Jin,&Fu Huihui.(2018).High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta).JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(5),1806-1811.
MLA Wu Chunhui,et al."High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.5(2018):1806-1811.
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