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| Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways | |
Yang, Yue1,2,3,4; Xing, Ronge1,2,4 ; Liu, Song1,2,4; Qin, Yukun1,2,4; Li, Kecheng1,2,4; Yu, Huahua1,2,4; Li, Pengcheng1,2,4
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| 2019-02-01 | |
| 发表期刊 | CARBOHYDRATE POLYMERS
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| ISSN | 0144-8617 |
| 卷号 | 205页码:401-409 |
| 通讯作者 | Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn) |
| 摘要 | Hydroxypropyltrimethyl ammonium chloride chitosan (HACC) is a water-soluble derivative of chitosan. To investigate the immunostimulatory effects of HACC, quaternized chitosans with different molecular weights were prepared and their effects on RAW 264.7 macrophages were compared. The results showed that HACC promoted nitric oxide (NO) production in a molecular weight- and dose-dependent manner. Lower molecular weight HACC was more active in promoting NO production. Furthermore, flow cytometry analysis showed that HACC significantly promoted the production of interleukin-6 and tumor necrosis factor-alpha. These results were further demonstrated by quantitive real-time reverse transcription polymerase chain reaction and western blot analysis. Moreover, western blotting revealed that HACC induced the phosphorylation of extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38, and signal transducer and activator of transcription (STAT) proteins. In conclusion, HACC activated RAW 264.7 cells through the mitogen-activated protein kinases and Janus kinase/STAT pathways. |
| 关键词 | Hydroxypropyltrimethyl ammonium chloride chitosan Immunostimulatory activity ERK JNK P38 STAT |
| DOI | 10.1016/j.carbpol.2018.10.101 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key R&D Program of China[2018YFC0311305] ; Special projects of Foshan science and technology innovation team[2017IT100054] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Key Research and Development Program of Shandong Province[2017YYSP018] ; National Key R&D Program of China[2018YFC0311305] ; Special projects of Foshan science and technology innovation team[2017IT100054] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] ; Key Research and Development Program of Shandong Province[2017YYSP018] |
| WOS研究方向 | Chemistry ; Polymer Science |
| WOS类目 | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| WOS记录号 | WOS:000450093200046 |
| 出版者 | ELSEVIER SCI LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/156391 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Xing, Ronge; Li, Pengcheng |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 第一作者单位 | 中国科学院海洋研究所 |
| 通讯作者单位 | 中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | Yang, Yue,Xing, Ronge,Liu, Song,et al. Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways[J]. CARBOHYDRATE POLYMERS,2019,205:401-409. |
| APA | Yang, Yue.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2019).Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways.CARBOHYDRATE POLYMERS,205,401-409. |
| MLA | Yang, Yue,et al."Hydroxypropyltrimethyl ammonium chloride chitosan activates RAW 264.7 macrophages through the MAPK and JAK-STAT signaling pathways".CARBOHYDRATE POLYMERS 205(2019):401-409. |
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| Hydroxypropyltrimeth（1085KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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