IOCAS-IR
Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance
Jia, Dongjie1; Fan, Lianmei1; Shen, Junling1; Qin, Song2; Li, Fuchao2; Yuan, Yongbing1
2019-01-03
Source PublicationSCIENTIA HORTICULTURAE
ISSN0304-4238
Volume243Pages:428-433
Corresponding AuthorYuan, Yongbing(yyb@qau.edu.cn)
AbstractApples may suffer sunburn during growing season because of photooxidation damage. Astaxanthin have a stronger antioxidant activity against photooxidation compared to its precursors zeaxanthin and beta-carotene. Here, we transferred beta-carotene ketolase (bkt) and beta-carotene hydroxylase (crtR-B), two genes encoding key enzymes for astaxanthin biosynthesis, into "Brookfield Gala" apple plants using pCAMBIA1302-bkt-crtR-B via transformation mediated by Agrobacterium tumefaciens EHA105. We aimed to confer a stronger antioxidant ability against sunburn to apple plants by regulating metabolic pathways of carotenoids. RT-PCR and qPCR analysis confirmed that bkt and crtR-B genes were expressed simultaneously in transgenic plants. Southern blotting analysis confirmed that the transgene was stably integrated and determined transgene copy number. Transgenic plants synthesized novel ketocarotenoids, resulting in an accumulation of astaxanthin up to 12.06 mu g/g and of canthaxanthin up to 6.38 mu g/g per leaf fresh weight. The light antioxidant ability of transgenic plantlets was stronger than that of non-transgenic ones in high light conditions. We speculate that the transgenic apples accumulating astaxanthin are resistant to photooxidation and can therefore prevent sunburn.
KeywordMalus domestica Borkh Astaxanthin beta-Carotene ketolase beta-Carotene hydroxylase Photoprotection
DOI10.1016/j.scienta.2018.08.058
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFD0400100] ; project of key agricultural technique research of Shandong Province ; [NSFC30471200]
WOS Research AreaAgriculture
WOS SubjectHorticulture
WOS IDWOS:000450381800053
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156386
Collection中国科学院海洋研究所
Corresponding AuthorYuan, Yongbing
Affiliation1.Qingdao Agr Univ, Coll Hort, Qingdao 266109, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Jia, Dongjie,Fan, Lianmei,Shen, Junling,et al. Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance[J]. SCIENTIA HORTICULTURAE,2019,243:428-433.
APA Jia, Dongjie,Fan, Lianmei,Shen, Junling,Qin, Song,Li, Fuchao,&Yuan, Yongbing.(2019).Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance.SCIENTIA HORTICULTURAE,243,428-433.
MLA Jia, Dongjie,et al."Genetic transformation of the astaxanthin biosynthetic genes bkt and crtR-B into apple tree to increase photooxidation resistance".SCIENTIA HORTICULTURAE 243(2019):428-433.
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