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Quantitative Proteomics Reveals Common and Specific Responses o a Marine Diatom Thalassiosira pseudonana to Different Macronutrient Deficiencies
Chen, Xiao-Huang1; Li, Yuan-Yuan1; Zhang, Hao1; Liu, Jiu-Ling1; Xie, Zhang-Xian1; Lin, Lin1; Wang, Da-Zhi1,2
2018-11-14
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume9Pages:20
Corresponding AuthorWang, Da-Zhi(dzwang@xmu.edu.cn)
AbstractMacronutrients such as nitrogen (N), phosphorus (P), and silicon (Si) are essential for the productivity and distribution of diatoms in the ocean. Responses of diatoms to a particular macronutrient deficiency have been investigated, however, we know little about their common or specific responses to different macronutrients. Here, we investigated the physiology and quantitative proteomics of a diatom Thalassiosira pseudonana grown in nutrient-replete, N-, P-, and Si-deficient conditions. Cell growth was ceased in all macronutrient deficient conditions while cell volume and cellular C content under P- and Si-deficiencies increased. Contents of chlorophyll a, protein and cellular N decreased in both N- and P-deficient cells but chlorophyll a and cellular N increased in the Si-deficient cells. Cellular P content increased under N-and Si-deficiencies. Proteins involved in carbon fixation and photorespiration were down-regulated under all macronutrient deficiencies while neutral lipid synthesis and carbohydrate accumulation were enhanced. Photosynthesis, chlorophyll biosynthesis, and protein biosynthesis were down-regulated in both N- and P-deficient cells, while Si transporters, light-harvesting complex proteins, chloroplastic ATP synthase, plastid transcription and protein synthesis were up-regulated in the Si-deficient cells. Our results provided insights into the common and specific responses of T. pseudonana to different macronutrient deficiencies and identified specific proteins potentially indicating a particular macronutrient deficiency.
Keywordmarine diatom Thalassiosira pseudonana macronutrient nitrogen phosphorus silicon quantitative proteomics
DOI10.3389/fmicb.2018.02761
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41230961] ; National Natural Science Foundation of China[41425021] ; National Key R&D Program of China[2017YFC1404300] ; Ten Thousand Talents Program for leading talents in science and technological innovation
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000450087500001
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156374
Collection海洋生态与环境科学重点实验室
Corresponding AuthorWang, Da-Zhi
Affiliation1.Xiamen Univ, State Key Lab Marine Environm Sci, Coll Environm & Ecol, Xiamen, Peoples R China
2.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao, Peoples R China
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Xiao-Huang,Li, Yuan-Yuan,Zhang, Hao,et al. Quantitative Proteomics Reveals Common and Specific Responses o a Marine Diatom Thalassiosira pseudonana to Different Macronutrient Deficiencies[J]. FRONTIERS IN MICROBIOLOGY,2018,9:20.
APA Chen, Xiao-Huang.,Li, Yuan-Yuan.,Zhang, Hao.,Liu, Jiu-Ling.,Xie, Zhang-Xian.,...&Wang, Da-Zhi.(2018).Quantitative Proteomics Reveals Common and Specific Responses o a Marine Diatom Thalassiosira pseudonana to Different Macronutrient Deficiencies.FRONTIERS IN MICROBIOLOGY,9,20.
MLA Chen, Xiao-Huang,et al."Quantitative Proteomics Reveals Common and Specific Responses o a Marine Diatom Thalassiosira pseudonana to Different Macronutrient Deficiencies".FRONTIERS IN MICROBIOLOGY 9(2018):20.
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