Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii

doi:10.1021/acs.jproteome.8b00568

IOCAS-IR > 实验海洋生物学重点实验室

	Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii
	Li, Rongfeng 1,2,3; Yu, Huahua1,2,3 ; Yue, Yang1,2,3 ; Li, Pengcheng1,2,3
	2018-11-01
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	17 期号:11 页码:3904-3913
通讯作者	Li, Rongfeng(rongfengli@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
摘要	Jellyfish are a type of poisonous cnidarian invertebrate that secrete lethal venom for predation or defense. Human beings often become victims of jellyfish stings accidentally while swimming or fishing and suffer severe pain, itching, swelling, inflammation, shock, and even death. Jellyfish venom is composed of various toxins, and the lethal toxin is the most toxic and hazardous component of the venom, which is responsible for deaths caused by jellyfish stings and envenomation. Our previous study revealed many toxins in jellyfish venom, including phospholipase A2, metalloproteinase, and protease inhibitors. However, it is still unknown which type of toxin is lethal and how it works. Herein a combined toxicology analysis, proteome strategy, and purification approach was employed to investigate the lethality of the venom of the jellyfish Cyanea nozakii. Toxicity analysis revealed that cardiotoxicity including acute myocardial infarction and a significant decrease in both heart rate and blood pressure is the primary cause of death. Purified lethal toxin containing a fraction of jellyfish venom was subsequently subjected to proteome analysis and bioinformation analysis. A total of 316 and 374 homologous proteins were identified, including phospholipase A2-like toxins and metalloprotease-like toxins. Furthermore, we confirmed that the lethality of the jellyfish venom is related to metalloproteinase activity but without any phospholipase A2 activity or hemolytic activity. Altogether, this study not only provides a comprehensive understanding of the lethal mechanism of jellyfish venom but also provides very useful information for the therapeutic or rescue strategy for severe jellyfish stings.
关键词	proteome jellyfish Cyanea nozakii venom CnV CnLF CnTX-1 CnTX-2
DOI	10.1021/acs.jproteome.8b00568
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876164] ; National Natural Science Foundation of China[41776163] ; National Key RD Plan[2017YFE0111100-04] ; Key Research and Development Plan of Shandong Province[2017GHY215003] ; Qingdao Science and Technology Project[17-3-3-21 nsh] ; National Natural Science Foundation of China[41876164] ; National Natural Science Foundation of China[41776163] ; National Key RD Plan[2017YFE0111100-04] ; Key Research and Development Plan of Shandong Province[2017GHY215003] ; Qingdao Science and Technology Project[17-3-3-21 nsh]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000449443000026
出版者	AMER CHEMICAL SOC
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/156346
专题	实验海洋生物学重点实验室
通讯作者	Li, Rongfeng; Li, Pengcheng
作者单位	1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Biol Prod, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Rongfeng,Yu, Huahua,Yue, Yang,et al. Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii[J]. JOURNAL OF PROTEOME RESEARCH,2018,17(11):3904-3913.
APA	Li, Rongfeng,Yu, Huahua,Yue, Yang,&Li, Pengcheng.(2018).Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii.JOURNAL OF PROTEOME RESEARCH,17(11),3904-3913.
MLA	Li, Rongfeng,et al."Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii".JOURNAL OF PROTEOME RESEARCH 17.11(2018):3904-3913.

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