Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line

doi:10.1016/j.scienta.2018.08.020

IOCAS-IR > 实验海洋生物学重点实验室

	Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line
	Liu, Qianchun 1,2,3; Wen, Changlong 1; Zhao, Hong 1; Wang, Yongqin 1
	2019-01-03
发表期刊	SCIENTIA HORTICULTURAE
ISSN	0304-4238
卷号	243 页码:101-106
通讯作者	Wang, Yongqin(wangyongqin@nercv.org)
摘要	Cytoplasmic male sterility (CMS) is a maternally inherited trait characterized by the inability to produce functional pollen and is important for exploiting heterosis in crop plants, but the molecular mechanisms of CMS and fertility restoration remain poorly understood in Welsh onion. Some CMS-associated genes were previously identified by RNA-Seq as candidate genes mediating male sterility in Welsh onion. In this study, the ATPase isoenzyme and ATPase activity were investigated. We also performed 2-dimensional electrophoresis to screen for ATPase-related proteins. The ATP synthase subunit alpha was found to be specifically expressed in the CMS line. Furthermore, we found that the full length of the atpA coding sequence was the same length in the CMS line and its maintainer line, but single nucleotide polymorphisms (SNPs) that lead to amino acid substitutions in the translated proteins were found. We hypothesize that the atpA gene is involved in regulating the development of CMS.
关键词	Welsh onion Cytoplasmic male sterility (CMS) atpA gene
DOI	10.1016/j.scienta.2018.08.020
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31372066] ; National Natural Science Foundation of China[31572119] ; Science and Technology Innovation Capability Construction of the Beijing Academy of Agriculture and Forestry Sciences[KJCX20170102] ; National Natural Science Foundation of China[31372066] ; National Natural Science Foundation of China[31572119] ; Science and Technology Innovation Capability Construction of the Beijing Academy of Agriculture and Forestry Sciences[KJCX20170102]
WOS研究方向	Agriculture
WOS类目	Horticulture
WOS记录号	WOS:000450381800013
出版者	ELSEVIER SCIENCE BV
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/156337
专题	实验海洋生物学重点实验室
通讯作者	Wang, Yongqin
作者单位	1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Liu, Qianchun,Wen, Changlong,Zhao, Hong,et al. Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line[J]. SCIENTIA HORTICULTURAE,2019,243:101-106.
APA	Liu, Qianchun,Wen, Changlong,Zhao, Hong,&Wang, Yongqin.(2019).Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line.SCIENTIA HORTICULTURAE,243,101-106.
MLA	Liu, Qianchun,et al."Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line".SCIENTIA HORTICULTURAE 243(2019):101-106.