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Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line
Liu, Qianchun1,2,3; Wen, Changlong1; Zhao, Hong1; Wang, Yongqin1
2019-01-03
Source PublicationSCIENTIA HORTICULTURAE
ISSN0304-4238
Volume243Pages:101-106
Corresponding AuthorWang, Yongqin(wangyongqin@nercv.org)
AbstractCytoplasmic male sterility (CMS) is a maternally inherited trait characterized by the inability to produce functional pollen and is important for exploiting heterosis in crop plants, but the molecular mechanisms of CMS and fertility restoration remain poorly understood in Welsh onion. Some CMS-associated genes were previously identified by RNA-Seq as candidate genes mediating male sterility in Welsh onion. In this study, the ATPase isoenzyme and ATPase activity were investigated. We also performed 2-dimensional electrophoresis to screen for ATPase-related proteins. The ATP synthase subunit alpha was found to be specifically expressed in the CMS line. Furthermore, we found that the full length of the atpA coding sequence was the same length in the CMS line and its maintainer line, but single nucleotide polymorphisms (SNPs) that lead to amino acid substitutions in the translated proteins were found. We hypothesize that the atpA gene is involved in regulating the development of CMS.
KeywordWelsh onion Cytoplasmic male sterility (CMS) atpA gene
DOI10.1016/j.scienta.2018.08.020
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31372066] ; National Natural Science Foundation of China[31572119] ; Science and Technology Innovation Capability Construction of the Beijing Academy of Agriculture and Forestry Sciences[KJCX20170102]
WOS Research AreaAgriculture
WOS SubjectHorticulture
WOS IDWOS:000450381800013
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156337
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Yongqin
Affiliation1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Minist Agr, Beijing 100097, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Qianchun,Wen, Changlong,Zhao, Hong,et al. Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line[J]. SCIENTIA HORTICULTURAE,2019,243:101-106.
APA Liu, Qianchun,Wen, Changlong,Zhao, Hong,&Wang, Yongqin.(2019).Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line.SCIENTIA HORTICULTURAE,243,101-106.
MLA Liu, Qianchun,et al."Comparative analysis of male sterility associated ATPase isoenzymes and atpA genes in a Welsh onion (Allium fistulosurn L.) cytoplasmic male sterility line and its maintainer line".SCIENTIA HORTICULTURAE 243(2019):101-106.
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