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A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas
Liu, Rui1; Cheng, Qi1,4; Song, Xiaorui2,3,5; Wang, Hao1; Wang, Xiudan1; Wang, Lingling3,5; Zhu, Beiwei4; Song, Linsheng2,3,5
2019-02-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume91Pages:132-142
Corresponding AuthorWang, Hao() ; Song, Linsheng(lshsong@dlou.edu.cn)
AbstractAs an important post-translational protein modification, ubiquitination has been demonstrated to play vitalrole in immune response of vertebrates. Ubiquitin (Ub)-conjugating enzyme E2 is the "heart" of ubiquitination, which is responsible for Ub cellular signaling and substrate modification. In the present study, an Ub-conjugating enzyme E2 (designed as CgUbe2g1) was identified from oyster Crassostrea gigas, and its regulation in the immune response against lipopolysaccharide (LPS) stimulation was investigated. CgUbe2g1 encoded a polypeptide of 168 amino acids with the predicted molecular mass of 19.20 kDa and contained conserved catalytic 'Ube domains. It shared a higher similarity with the known UBC2G1 type E2s and was closely clustered with the type E2s identified from invertebrates in the phylogenetic assay. The mRNA transcripts of CgUbe2g1 were mainly distributed in hemocyte, mantle, hepatopancreas and male gonad of C. gigas. CgUbe2g1 protein was found to be colocalized with Ub around the nucleus of oyster hemocyte. The recombinant CgUbe2g1 protein (rCgUbe2g1) could activate the ubiquitination in vitro by binding both activated and un-activated Ub. The expressions of inflammation-related factors TNF-alpha and NF-kappa B in CgUbe2g1 transfected cells were both significantly up-regulated after LPS stimulation, which were 12.9-fold at 3h (p < 0.01) and 2.3-fold at 6h (p < 0.01) of that in negative control group, respectively. The phagocytic rate of hemocyte and the ROS level in hemocyte were both significantly decreased (p < 0.01), while the apoptosis rate was significantly increased (p < 0.01) after CgUbe2g1 mRNA was interfered. These results demonstrated that Ub-conjugating enzyme CgUbe2g1 was involved in the innate immune response of oyster against invading pathogen, which might play important roles in the activation of inflammatory response and regulation of cellular immune response.
KeywordUb-conjugating enzyme E2 Inflammatory response Cellular immunity Crassostrea gigas NF-kappa B pathway Ubiquitination
DOI10.1016/j.dci.2018.10.014
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation of China[U1706204] ; Aoshan Talent Cultivation Program - Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Modern Agro-industry Technology Research System, Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture[CARS-49] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; Dalian High Level Talent Innovation Support Program[2015R020]
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000451492900015
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156149
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Hao; Song, Linsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Dalian Univ Technol, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
4.Dalian Polytech Univ, Sch Food Sci & Technol, Dalian 116034, Peoples R China
5.Dalian Univ Technol, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Liu, Rui,Cheng, Qi,Song, Xiaorui,et al. A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,91:132-142.
APA Liu, Rui.,Cheng, Qi.,Song, Xiaorui.,Wang, Hao.,Wang, Xiudan.,...&Song, Linsheng.(2019).A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,91,132-142.
MLA Liu, Rui,et al."A vital ubiquitin-conjugating enzyme CgUbe2g1 participated in regulation of immune response of Pacific oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 91(2019):132-142.
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