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The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas
Wang, Lingling1; Zhang, Huan2; Wang, Mengqiang3; Zhou, Zhi3; Wang, Weilin1; Liu, Rui3; Huang, Mengmeng3; Yang, Chuanyan1; Qiu, Limei3; Song, Linsheng1,3
2019-02-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume91Pages:1-7
Corresponding AuthorZhang, Huan(zhanghuan@qdio.ac.cn) ; Song, Linsheng(lshsong@dlou.edu.cn)
AbstractPattern recognition receptors (PRRs) are essential in recognizing specific pathogen-associated molecular patterns (PAMPs) on microbes and triggering responses to eliminate the invading pathogens. Previous genomic studies have revealed a great number of PRR genes in the Pacific oyster Crassostrea gigas, a sessile and filter-feeder marine bivalve belonging to the phylum Mollusca. On the survey of PRRs in the assembly oyster reference genome version 9, a total of 1084 PRRs were identified, which were composed of at least 12 gene families. Some of the gene families were significantly expanded, including C-type lectins (CTLs), fibrinogen-related proteins (FREPs), scavenger receptor cysteine-rich repeat protein (SRCRs), leucine-rich repeat (LRR)-only proteins (LRRops), and especially Clq domain-containing proteins (ClqDCs). The transcriptomic profiles of these abundant PRRs in response to PAMP treatments were investigated by RNA-Seq using the SOLiD EZ BeadTM system. Compared to the control library, there were 6,655, 7,273, 7,593, 6,830, 6687 and 8250 differentially expressed genes in the haemocytes of oysters in response to lipopolysaccharide (LPS) stimulation for 6 h, 12 h and 24 h, and peptidoglycan (PGN), glucan (GLU) and poly I:C (IC) stimulation for 12 h, respectively. After stimulation for 12 h, there were 134, 97, 114 and 159 genes up-regulated in the LPS, PGN, GLU and IC library, respectively. Most of the gene families involved in immune response towards PAMPs were ClqDCs, CTLs and FREPs, while only a few members of LRR and immunoglobin-containing proteins (LRRIGs), retinoic acid-inducible gene I [RIG-I]-like receptors (RLRs) and Toll like receptors (TLRs) were up-regulated. After LPS stimulation, the expression level of 258 non-redundant PRR genes in oyster haemocytes increased significantly with different expression pattern, and most of them were ClqDCs, CTLs, LRRops and FREPs. The transcriptomic analyses indicated that there was a dynamic and orchestrated specific expression regulation of numerous PRR genes in response to pathogen invasion. The expanded PRR gene family members were differentiated with more specific functional responses to certain PAMPs rather than the versatile ones. Based on the different expression pattern during the LPS stimulation, the oyster PRRs could be assigned into three consecutive steps in the response against pathogen invading. All the results would provide useful information for future studies of oyster PRRs and deep insight into the researches on invertebrate innate immunity.
KeywordBivalve immunity Pattern recognition receptors Crassostrea gigas Transcriptome RNA-Seq
DOI10.1016/j.dci.2018.09.021
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation of China[U1706204] ; National Science Foundation of China[41476145] ; Modern Agro-industry Technology Research System[CARS-49] ; Outstanding Talents and Innovative Teams of Agricultural Scientific Research in the Ministry of Agriculture, Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Distinguished Professor in Liaoning ; Dalian Ocean University
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000451492900001
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156145
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Huan; Song, Linsheng
Affiliation1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Corresponding Author AffilicationChinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res;  Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Lingling,Zhang, Huan,Wang, Mengqiang,et al. The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,91:1-7.
APA Wang, Lingling.,Zhang, Huan.,Wang, Mengqiang.,Zhou, Zhi.,Wang, Weilin.,...&Song, Linsheng.(2019).The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,91,1-7.
MLA Wang, Lingling,et al."The transcriptomic expression of pattern recognition receptors: Insight into molecular recognition of various invading pathogens in Oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 91(2019):1-7.
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