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Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus
Duan Hu1; Jin Songjun1; Li Fuhua1,2; Zhang Xiaojun1,2; Xiang Jianhai1,2
2018-11-01
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume36Issue:6Pages:2368-2378
Corresponding AuthorXiang Jianhai(jhxiang@qdio.ac.cn)
AbstractAs in vertebrates, brains play key roles in rhythmic regulation, neuronal maintenance, differentiation and function, and control of the release of hormones in arthropods. But the structure and functional domains of the brain are still not very clear in crustaceans. In the present study, we reveal the structural details of the brain in the redclaw crayfish using hematoxylin-eosin staining and microscopic examination, firstly. The brain of crayfish is consist of three main parts, namely, protocerebrum, deutocerebrum, and tritocerebrum, including some tracts and commissures, briefly. Secondly, at least 9 kinds of brain cells were identified on the basis of topology and cell shapes, as well as antibody labeling. We also provide morphological details of most cell types, which were previously un-described. In general, four types of glia and three types of neurosecretory cells were described except cluster 9/11 and cluster 10 cells. Glia were categorized into another three main kinds: (1) surface glia; (2) cortex glia; and (3) neuropile glia in addition to astrocytes identified by GFAP labelling. And neurosecretory cells were categorized into I, II and III types based on morphological observation. Finally, cluster 9/11 and 10 cells derived from the brain of crayfish, could be used for primary culture about 7-9 d under the optimized conditions. There results provide a resource for improving the knowledge of the still incompletely defined neuroendocrinology of this species. Using the crayfish as an animal model, we are easy to carry out further research in manipulating their endocrine system, exploring cellular and synaptic mechanisms so much as larval production on a small scale, such as in a cell or tissue.
KeywordCherax quadricarinatus brain's structure brain cells primary culture
DOI10.1007/s00343-019-7145-x
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41376165] ; National Natural Science Foundation of China-Israel Science Foundation (NSFC-ISF)[31461143007] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000454629000039
PublisherSCIENCE PRESS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155954
Collection实验海洋生物学重点实验室
Corresponding AuthorXiang Jianhai
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Duan Hu,Jin Songjun,Li Fuhua,et al. Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(6):2368-2378.
APA Duan Hu,Jin Songjun,Li Fuhua,Zhang Xiaojun,&Xiang Jianhai.(2018).Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(6),2368-2378.
MLA Duan Hu,et al."Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.6(2018):2368-2378.
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