Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus

doi:10.1007/s00343-019-7145-x

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	Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus
	Duan Hu 1; Jin Songjun 1; Li Fuhua 1,2; Zhang Xiaojun 1,2; Xiang Jianhai1,2
	2018-11-01
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
卷号	36 期号:6 页码:2368-2378
通讯作者	Xiang Jianhai(jhxiang@qdio.ac.cn)
摘要	As in vertebrates, brains play key roles in rhythmic regulation, neuronal maintenance, differentiation and function, and control of the release of hormones in arthropods. But the structure and functional domains of the brain are still not very clear in crustaceans. In the present study, we reveal the structural details of the brain in the redclaw crayfish using hematoxylin-eosin staining and microscopic examination, firstly. The brain of crayfish is consist of three main parts, namely, protocerebrum, deutocerebrum, and tritocerebrum, including some tracts and commissures, briefly. Secondly, at least 9 kinds of brain cells were identified on the basis of topology and cell shapes, as well as antibody labeling. We also provide morphological details of most cell types, which were previously un-described. In general, four types of glia and three types of neurosecretory cells were described except cluster 9/11 and cluster 10 cells. Glia were categorized into another three main kinds: (1) surface glia; (2) cortex glia; and (3) neuropile glia in addition to astrocytes identified by GFAP labelling. And neurosecretory cells were categorized into I, II and III types based on morphological observation. Finally, cluster 9/11 and 10 cells derived from the brain of crayfish, could be used for primary culture about 7-9 d under the optimized conditions. There results provide a resource for improving the knowledge of the still incompletely defined neuroendocrinology of this species. Using the crayfish as an animal model, we are easy to carry out further research in manipulating their endocrine system, exploring cellular and synaptic mechanisms so much as larval production on a small scale, such as in a cell or tissue.
关键词	Cherax quadricarinatus brain's structure brain cells primary culture
DOI	10.1007/s00343-019-7145-x
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41376165] ; National Natural Science Foundation of China-Israel Science Foundation (NSFC-ISF)[31461143007] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Natural Science Foundation of China[41376165] ; National Natural Science Foundation of China-Israel Science Foundation (NSFC-ISF)[31461143007] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000454629000039
出版者	SCIENCE PRESS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/155953
专题	实验海洋生物学重点实验室
通讯作者	Xiang Jianhai
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Duan Hu,Jin Songjun,Li Fuhua,et al. Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(6):2368-2378.
APA	Duan Hu,Jin Songjun,Li Fuhua,Zhang Xiaojun,&Xiang Jianhai.(2018).Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(6),2368-2378.
MLA	Duan Hu,et al."Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.6(2018):2368-2378.

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