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Characterization and expression of androgen receptors in olive flounder
Zou, Yuxia1,2,4; Peng, Limin1,3; Weng, Shenda1,3; Liang, Dongdong1,2,3; Fan, Zhaofei1,2,3; Wu, Zhihao1,2,4; Tan, Xungang1,2,4; Jiao, Shuang1,2,4; You, Feng1,2,4
2019-01-30
发表期刊GENE
ISSN0378-1119
卷号683页码:184-194
通讯作者You, Feng(youfeng@qdio.ac.cn)
摘要Androgens are critical hormones that regulate sex differentiation, sexual maturation, and spermatogenesis in vertebrates, which is mainly mediated by androgen receptors (ARs). Reports on transcript variants of ar (AR encoding gene) in human are almost always associated with cancers and androgen insensitivity syndrome. However, the knowledge of ar variants in teleosts is scarce. In this study, ar beta and two transcript variants of ar alpha (ar alpha 1 and ar alpha 2) in olive flounder (Paralichthys olivaceus) were cloned and analyzed. Their expression patterns were investigated in 16 adult female and male tissues by RT-PCR, respectively. aral was expressed in the majority of tissues excluding male liver, medulla oblongata and female cerebellum, with higher levels in male gonad, kidney, head kidney, intestine, stomach, spleen, heart and gill than in female. ar alpha 2 had similar expression patterns as ar alpha 1, with lower levels in general. ar beta was also widely expressed in various tissues excluding male spleen, female spleen and gill, with higher levels in male gonad, kidney, head kidney, intestine and lower levels in hypothalamus than in female. Compared with ar beta, much lower expression levels of ar alpha 1 and ar alpha 2 were detected in different brain areas. The real-time quantitative PCR (qPCR) results showed that the total ara expression level was relatively higher during olive flounder gonadal differentiation and before the onset of testis differentiation, whereas ar beta was expressed significantly higher during male gonadal differentiation period than female gonadal differentiation period. The in vitro transient transfection assays showed that AR alpha 1, AR alpha 2 and AR beta could all suppress the activity of cyp19 alpha (p450arom aromatase gene) promoter, and the inhibitory effect of AR alpha 1 was dose dependent. Our results imply that ar alpha 1, ar alpha 2 and ar beta are sex-related genes and they might play important roles in gonadal differentiation in flounder.
关键词Androgen receptor Transcript variant Gene expression Gene regulation Gonadal differentiation Paralichthys olivaceus
DOI10.1016/j.gene.2018.10.018
收录类别SCI
语种英语
资助项目National Natural Science Foundation of China[41276171] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470] ; National Natural Science Foundation of China[41276171] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS研究方向Genetics & Heredity
WOS类目Genetics & Heredity
WOS记录号WOS:000452565700021
出版者ELSEVIER SCIENCE BV
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qdio.ac.cn/handle/337002/155823
专题实验海洋生物学重点实验室
通讯作者You, Feng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
通讯作者单位中国科学院海洋研究所;  中国科学院海洋大科学研究中心
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GB/T 7714
Zou, Yuxia,Peng, Limin,Weng, Shenda,et al. Characterization and expression of androgen receptors in olive flounder[J]. GENE,2019,683:184-194.
APA Zou, Yuxia.,Peng, Limin.,Weng, Shenda.,Liang, Dongdong.,Fan, Zhaofei.,...&You, Feng.(2019).Characterization and expression of androgen receptors in olive flounder.GENE,683,184-194.
MLA Zou, Yuxia,et al."Characterization and expression of androgen receptors in olive flounder".GENE 683(2019):184-194.
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