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Characterization and expression of androgen receptors in olive flounder
Zou, Yuxia1,2,4; Peng, Limin1,3; Weng, Shenda1,3; Liang, Dongdong1,2,3; Fan, Zhaofei1,2,3; Wu, Zhihao1,2,4; Tan, Xungang1,2,4; Jiao, Shuang1,2,4; You, Feng1,2,4
2019-01-30
Source PublicationGENE
ISSN0378-1119
Volume683Pages:184-194
Corresponding AuthorYou, Feng(youfeng@qdio.ac.cn)
AbstractAndrogens are critical hormones that regulate sex differentiation, sexual maturation, and spermatogenesis in vertebrates, which is mainly mediated by androgen receptors (ARs). Reports on transcript variants of ar (AR encoding gene) in human are almost always associated with cancers and androgen insensitivity syndrome. However, the knowledge of ar variants in teleosts is scarce. In this study, ar beta and two transcript variants of ar alpha (ar alpha 1 and ar alpha 2) in olive flounder (Paralichthys olivaceus) were cloned and analyzed. Their expression patterns were investigated in 16 adult female and male tissues by RT-PCR, respectively. aral was expressed in the majority of tissues excluding male liver, medulla oblongata and female cerebellum, with higher levels in male gonad, kidney, head kidney, intestine, stomach, spleen, heart and gill than in female. ar alpha 2 had similar expression patterns as ar alpha 1, with lower levels in general. ar beta was also widely expressed in various tissues excluding male spleen, female spleen and gill, with higher levels in male gonad, kidney, head kidney, intestine and lower levels in hypothalamus than in female. Compared with ar beta, much lower expression levels of ar alpha 1 and ar alpha 2 were detected in different brain areas. The real-time quantitative PCR (qPCR) results showed that the total ara expression level was relatively higher during olive flounder gonadal differentiation and before the onset of testis differentiation, whereas ar beta was expressed significantly higher during male gonadal differentiation period than female gonadal differentiation period. The in vitro transient transfection assays showed that AR alpha 1, AR alpha 2 and AR beta could all suppress the activity of cyp19 alpha (p450arom aromatase gene) promoter, and the inhibitory effect of AR alpha 1 was dose dependent. Our results imply that ar alpha 1, ar alpha 2 and ar beta are sex-related genes and they might play important roles in gonadal differentiation in flounder.
KeywordAndrogen receptor Transcript variant Gene expression Gene regulation Gonadal differentiation Paralichthys olivaceus
DOI10.1016/j.gene.2018.10.018
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41276171] ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000452565700021
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155823
Collection实验海洋生物学重点实验室
Corresponding AuthorYou, Feng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zou, Yuxia,Peng, Limin,Weng, Shenda,et al. Characterization and expression of androgen receptors in olive flounder[J]. GENE,2019,683:184-194.
APA Zou, Yuxia.,Peng, Limin.,Weng, Shenda.,Liang, Dongdong.,Fan, Zhaofei.,...&You, Feng.(2019).Characterization and expression of androgen receptors in olive flounder.GENE,683,184-194.
MLA Zou, Yuxia,et al."Characterization and expression of androgen receptors in olive flounder".GENE 683(2019):184-194.
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