IOCAS-IR  > 海洋地质与环境重点实验室
Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China
Sun, Wuyang1,2; Cao, Wenrui3; Jiang, Mingyu3; Saren, Gaowa3; Liu, Jiwei1,2,4; Cao, Jiangfei4; Ali, Imran1,2; Yu, Xinke3; Peng, Changsheng1,2,4; Naz, Iffat5,6
2018
Source PublicationRSC ADVANCES
ISSN2046-2069
Volume8Issue:69Pages:39710-39720
Corresponding AuthorPeng, Changsheng(pcs005@ouc.edu.cn) ; Naz, Iffat(iffatkhattak@yahoo.com)
AbstractIn the present research investigation, 13 indigenous bacteria (from CQ1 to CQ13) were isolated from soil collected from Changqing oil field of Xi'an, China. Four promising biosurfactant producers (CQ1, CQ2, CQ4, and CQ13) were selected through primary screening among these 13 strains, including via drop collapse and oil-spreading methods. However, only the strain CQ2 showed the best biosurfactant production and was further screened by hemolytic assay, cetyl trimethyl ammonium bromide (CTAB), surface tension and emulsifying activity. The bacterium CQ2 has the ability to produce about 3.015 g L-1 of biosurfactant using glucose as the sole carbon source without any optimization. The produced biosurfactant could greatly reduce surface tension from 72.66 to 24.72 mN m(-1) with a critical micelle concentration (CMC) of 30 mg L-1 and emulsify diesel oil up to 60.1%. The cell-free broth was found to be stable in wide temperature (4-100 degrees C), pH (6-12) and salinity (2-20%) ranges for surface and emulsifying activity. This biosurfactant was preliminarily found to be of a glycolipid nature as evident from thin-layer chromatographic (TLC) and Fourier transform infra-red spectroscopic (FTIR) analyses. Moreover, CQ2 was able to degrade 54.7% of diesel oil, which surprisingly could form a substantial amount of bioflocculants during the degradation process. Furthermore, the 16S rDNA sequence using the Genbank BLAST tool revealed that isolated CQ2 was closely related to species of Pseudomonas genus and, thus, was entitled Pseudomonas sp. CQ2. The results of residual diesel oil contents measured by GC-MS showed that C7-C28 hydrocarbons could be degraded by Pseudomonas sp. CQ2. Thus, these findings revealed that CQ2 could be applied for remediation of diesel oil/petroleum-contaminated waters and soils on a large scale.
DOI10.1039/c8ra07721e
Indexed BySCI
Language英语
Funding ProjectState Key Laboratory of Environmental Criteria and Risk Assessment[SKLECRA2013FP12] ; Shandong Province Key Research and Development Program[2016GSF115040] ; National Nature Science Foundation of China[41406060]
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS IDWOS:000452116300054
PublisherROYAL SOC CHEMISTRY
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155814
Collection海洋地质与环境重点实验室
Corresponding AuthorPeng, Changsheng; Naz, Iffat
Affiliation1.Ocean Univ China, Key Lab Marine Environm Sci & Ecol, Minist Educ, Qingdao 266100, Peoples R China
2.Ocean Univ China, Coll Environm Sci & Engn, Qingdao 266100, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
4.Zhaoqing Univ, Sch Environm & Chem Engn, Zhaoqing 526061, Peoples R China
5.Qassim Univ, Deanship Educ Serv, Dept Biol, Buraydah 51452, Saudi Arabia
6.Quaid i Azam Univ, Dept Microbiol, Islamabad, Pakistan
Recommended Citation
GB/T 7714
Sun, Wuyang,Cao, Wenrui,Jiang, Mingyu,et al. Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China[J]. RSC ADVANCES,2018,8(69):39710-39720.
APA Sun, Wuyang.,Cao, Wenrui.,Jiang, Mingyu.,Saren, Gaowa.,Liu, Jiwei.,...&Naz, Iffat.(2018).Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China.RSC ADVANCES,8(69),39710-39720.
MLA Sun, Wuyang,et al."Isolation and characterization of biosurfactant-producing and diesel oil degrading Pseudomonas sp CQ2 from Changqing oil field, China".RSC ADVANCES 8.69(2018):39710-39720.
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