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Global discovery of small RNAs in the fish pathogen Edwardsiella piscicida: key regulator of adversity and pathogenicity
Du, He-he1,3; Zhou, Hai-Zhen4,5; Tang, Ping6; Huang, Hui-qin1,3; Liu, Min1,3; Hu, Yong-hua1,2,3
2018-12-11
Source PublicationVETERINARY RESEARCH
ISSN0928-4249
Volume49Pages:12
Corresponding AuthorLiu, Min(liuming@itbb.org.cn) ; Hu, Yong-hua(huyonghua@itbb.org.cn)
AbstractRecently, bacterial small RNA (sRNA) has been shown to be involved as a key regulator in stress responses. sRNAs of Edwardsiella piscicida, an important aquatic pathogen, are not well characterized to date. In this study, using RNA-seq technology, we globally found and identified sRNA candidates expressed from E. piscicida grown in normal LB medium, acid pressure, iron deficiency stress, and oxidation pressure. A total of 148 sRNAs were found, including 19 previously annotated sRNAs and 129 novel sRNA candidates by searching against the Rfam database. Compared in normal condition, the expression of 103 sRNAs (DEsRNA, differentially expressed sRNA) and 1615 mRNAs (DEmRNAs, differentially expressed mRNA) showed significant differences in three stress sample. Based on the prediction by IntaRNA and relational analysis between DEsRNAs and DEmRNAs, 103 DEsRNAs were predicted to regulate 769 target mRNAs. Pleiotropic function of target DEmRNAs indicated that sRNAs extensively participated in a variety of physiological processes, including response to adversity and pathogenicity, the latter was further confirmed by infection experiment. A large number transcription factors appeared in target genes of sRNAs, which suggested that sRNAs likely deeply interlaced within complex gene regulatory networks of E. piscicida. Moreover, 49 Hfq-associated sRNAs were also identified in this study. In summary, we globally discovered sRNAs for the first time in pathogenic bacteria of fish, and our findings indicated that sRNAs in E. piscicida have important roles in adaptation to environmental stress and pathogenicity. These results also provide clues for deciphering regulation mechanism of gene expression related to physiological response and pathogenicity.
DOI10.1186/s13567-018-0613-z
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41476138] ; Financial Fund of the Ministry of Agriculture and Rural Affairs, P. R. of China[NFZX2018] ; Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences[1630052018005]
WOS Research AreaVeterinary Sciences
WOS SubjectVeterinary Sciences
WOS IDWOS:000452912400001
PublisherBMC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/155758
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu, Min; Hu, Yong-hua
Affiliation1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Haikou 571101, Hainan, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Hainan Prov Key Lab Funct Components Res & Utiliz, Haikou 571101, Hainan, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Yunnan Agr Univ, Kunming 650200, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Du, He-he,Zhou, Hai-Zhen,Tang, Ping,et al. Global discovery of small RNAs in the fish pathogen Edwardsiella piscicida: key regulator of adversity and pathogenicity[J]. VETERINARY RESEARCH,2018,49:12.
APA Du, He-he,Zhou, Hai-Zhen,Tang, Ping,Huang, Hui-qin,Liu, Min,&Hu, Yong-hua.(2018).Global discovery of small RNAs in the fish pathogen Edwardsiella piscicida: key regulator of adversity and pathogenicity.VETERINARY RESEARCH,49,12.
MLA Du, He-he,et al."Global discovery of small RNAs in the fish pathogen Edwardsiella piscicida: key regulator of adversity and pathogenicity".VETERINARY RESEARCH 49(2018):12.
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